Human Laminin alpha 1 N-Terminus Domain VI Antibody Summary
Leu22-Met269
Accession # P25391
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Laminin alpha 1 in U2OS Human Cell Line by Flow Cytometry. U2OS human osteosarcoma cell line was stained with Goat Anti-Human Laminin alpha 1 Antigen Affinity-Purified Polyclonal Antibody (Catalog # AF4187, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by APC-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Laminin alpha 1
Laminin subunit alpha 1 (LAMA1) is a secreted 400 kDa extracellular matrix glycoprotein that contributes to the formation of basement membrane Laminin isoforms 1 and 3. It is one of three subunits ( alpha, beta, and gamma ) that interact via their coiled-coil domains to form the approximately 800 kDa cruciform, disulfide-linked, Laminin heterotrimer. The 3058 amino acid (aa) residue mature human alpha 1 chain contains an N-terminal Laminin VI domain (aa 18‑269), followed by domains V through III containing 17 EGF-like repeats, the coiled-coil domains II and I, and five globular, Laminin G-like domains. Over aa 22‑269, human Laminin alpha 1 shares 95% and 91% aa sequence identity with canine and mouse alpha 1 chain, respectively.
Product Datasheets
Citations for Human Laminin alpha 1 N-Terminus Domain VI Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Nerve pathology is prevented by linker proteins in mouse models for LAMA2-related muscular dystrophy
Authors: Judith R Reinhard, Emanuela Porrello, Shuo Lin, Pawel Pelczar, Stefano C Previtali, Markus A Rüegg
PNAS Nexus
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Characterization of Laminins in Healthy Human Aortic Valves and a Modified Decellularized Rat Scaffold
Authors: Carl Granath, Hunter Noren, Hanna Björck, Nancy Simon, Kim Olesen, Sergey Rodin et al.
BioResearch Open Access
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Influence of Human Platelet Lysate on Extracellular Matrix Deposition and Cellular Characteristics in Adipose-Derived Stem Cell Sheets
Authors: Nai-Chen Cheng, Yuan-Kun Tu, Ning-Hsu Lee, Tai-Horng Young
Frontiers in Cell and Developmental Biology
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Linker proteins restore basement membrane and correct LAMA2 -related muscular dystrophy in mice
Authors: Judith R. Reinhard, Shuo Lin, Karen K. McKee, Sarina Meinen, Stephanie C. Crosson, Maurizio Sury et al.
Science Translational Medicine
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