Human LAIR1 Antibody

Catalog #: AF2664 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2664
AF2664-SP

Detection of LAIR1 in PBMC lymphocytes by Flow Cytometry

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Human LAIR1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human LAIR1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human LAIR2 is observed and less than 2% cross-reactivity with recombinant mouse LAIR1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LAIR1 isoform 1
Gln22-His163
Accession # Q6GTX8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human LAIR1

Flow Cytometry

0.25 µg/10⁶ cells

Human PBMC lymphocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of LAIR1 in PBMC lymphocytes by Flow Cytometry PBMC lymphocytes were stained with Mouse Anti-Human CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB3791P) and either (A) Goat Anti-Human LAIR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # af2664) or (B) isotype control antibody (Catalog # AB-108-C) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LAIR1

Leukocyte-associated Ig-like receptor-1 (LAIR1; also CD305) is a 46 kDa inhibitory receptor belonging to the Ig superfamily. It is a type I transmembrane protein with one extracellular Ig-like domain and two cytoplasmic ITIMs. Four LAIR-1 splice variants exist. LAIR1b has a 17 aa deletion outside the Ig loop in the extracellular domain. It differs from LAIR1c by one aa residue. LAIR1d has a 77 aa truncation in the cytoplasmic domain. LAIR1 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells and most thymocytes. The extracellular domain of human LAIR1 shares 40% aa identity with that of the mouse protein.

Long Name

Leukocyte-associated Ig-like Receptor 1

Entrez Gene IDs

3903 (Human); 52855 (Mouse); 574531 (Rat); 102136964 (Cynomolgus Monkey)

Alternate Names

CD305 antigen; CD305; CD305leukocyte-associated Ig-like receptor 1; HLAIR1; LAIR1; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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