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Human KIR2DS4/CD158i PE-conjugated Antibody

Catalog #: FAB1847P
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1847P-025
FAB1847P-100
Detection of KIR2DS4/CD158i in Human PBMCs by Flow Cytometry.
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Human KIR2DS4/CD158i PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects KIR2DS4/CD158i in Western blots. In flow cytometry, this antibody stains cells transfected with KIR2DS4/CD158i. Does not stain cells transfected with KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 3DL1, 3DL2, or 3DS1.
Source
Monoclonal Mouse IgG2A Clone # 179315
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human KIR2DS4
Accession # P43632
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of KIR2DS4/CD158i antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of KIR2DS4/CD158i in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human KIR2DS4/CD158i PE‑conjugated Monoclonal Antibody (Catalog # FAB1847P) and Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: KIR2DS4/CD158i

KIR2DS4 is an activating immunoglobulin-like receptor expressed on NK cells and some T cells. It has been implicated in the recognition of some HLA-C alleles (1). A deletion variant encoding a single domain secreted isoform of KIR2DS4 has also been described (2).

References
  1. Katz, G. et al. (2001) J. Immunol. 166:7260.
  2. Maxwell, L. et al. (2002) Tissue Antigens 60:254.
Long Name
Killer Cell Immunoglobulin-like Receptor, Two Domains, Short Cytoplasmic Tail, 4
Entrez Gene IDs
3809 (Human)
Alternate Names
CD158 antigen-like family member I; CD158i antigen; CD158i; cl-39; killer cell immunoglobulin-like receptor 2DS4; killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail4,nkat8; killer inhibitory receptor 4-1-2; KIR antigen 2DS4; KIR2DS4; KKA3KIR1D; MGC120019; MGC125315; MGC125317; MHC class I NK cell receptor; natural killer cell inhibitory receptor; Natural killer-associated transcript 8; NKAT-8; NKAT8KIR412; P58 natural killer cell receptor clones CL-39/CL-17; p58 NK receptor CL-39/CL-17

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Citation for Human KIR2DS4/CD158i PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The Clinical Features of Patients with Chronic Hepatitis C Virus Infections Are Associated with Killer Cell Immunoglobulin-Like Receptor Genes and Their Expression on the Surface of Natural Killer Cells
    Authors: A Podhorzer, M Dirchwolf, A Machicote, S Belen, S Montal, S Paz, H Fainboim, LG Podestá, L Fainboim
    Front Immunol, 2018-01-05;8(0):1912.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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