Human Integrin beta 2/CD18 Antibody Summary
Gln23-Asn700
Accession # AAA59490
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Integrin beta 2/CD18 in Human PBMCs. Integrin beta 2/CD18 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human Integrin beta 2/CD18 Monoclonal Antibody (Catalog # MAB1730) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 2/CD18
Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or complement receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1‑7). Like other integrins, alpha X beta 2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alpha X beta 2 is fully active and extended. The alpha X vWFA or I-domain, which contains the adhesion sites, forms the N-terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain (1, 8). In the inactive state, the heterodimer flexes in the center at the alpha X thigh and calf domains and beta 2 I-EGF domains, impeding access to adhesion sites (1). The 1088 aa human alpha X/CD11c ECD shares 70‑76% aa sequence identity with mouse, rat and canine alpha X while the 678 aa human beta 2/CD18 ECD shares 81‑83% aa sequence identity with mouse, rat, cow, dog, goat, sheep, and pig beta 2. Potential alpha X isoforms containing 719 and 725 aa (as compared to full-length 1163 aa alpha X) lack the vWFA domain and the N-terminus. Active alpha X beta 2 shares some adhesion partners with alpha M beta 2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4‑11). However, alpha M beta 2 activity is often constitutive, while alpha X beta 2 activity requires cell activation (4‑7). alpha X beta 2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (11‑16). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 9, 11, 12, 16). Mutations of beta 2, especially in the vWFA domain, cause leukocyte adhesion deficiency (LAD-1) and susceptibility to bacterial infections (17).
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- Beyer, M. et al. (2005) Respir. Res. 6:70.
- Nicolaou, F. et al. (2003) Blood 101:4033.
- Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
- Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
- Pendu, R. et al. (2006) Blood 108:3746.
- Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
- Schack, L. et al. (2009) J. Immunol. 182:6943.
- Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
- Gang, J. et al. (2007) Mol. Cells 24:240.
- Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
- Vorup-Jensen, T. et al. (2004) Proc. Natl. Acad. Sci. USA 102:1614.
- Kishimoto, T.K. et al. (1987) Cell 50:193.
Product Datasheets
Citations for Human Integrin beta 2/CD18 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Label-free proteomics reveals decreased expression of CD18 and AKNA in peripheral CD4+ T cells from patients with Vogt-Koyanagi-Harada syndrome.
Authors: Mao L, Yang P, Hou S
PLoS ONE, 2011-01-28;6(1):e14616.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
The role of vascular cell adhesion molecule 1/ very late activation antigen 4 in endothelial progenitor cell recruitment to rheumatoid arthritis synovium.
Authors: Silverman MD, Haas CS, Rad AM, Arbab AS, Koch AE
Arthritis Rheum., 2007-06-01;56(6):1817-26.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology, 2004-06-01;112(2):274-89.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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