Human Immunotherapy Luminex® Performance Assay 25-plex Fixed Panel

Catalog #: LKTM010B Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
LKTM010B

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Summary Product Features Precision & Accuracy Data Examples Product Datasheets

Human Immunotherapy Luminex® Performance Assay 25-plex Fixed Panel Summary

Assay Type	bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and antibody-coated beads
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Simultaneously profile up to 50 analytes of your choice in one sample
Choose from a wide menu of analytes, including many unique analytes
Requires a small sample volume (<50 µL)
Run your assay in just 3-4 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Please allow additional time for international orders.

Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Standard/Sample Diluent
Wash Buffer
Streptavidin-PE
Foil Plate Sealers (4)
Mixing Bottle
Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

	n=40 (20x2 levels)	n=30
Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 30 different serum assays.
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
CD40 Ligand	3.8	10.8
GM-CSF	3.4	11.2
Granzyme B	2.9	8.2
IFN-alpha 2	3.6	10.0
IFN-gamma	2.3	10.9
IL-1 alpha	5.0	10.6
IL-1 beta	2.8	10.1
IL-1ra	3.6	12.4
IL-2	3.9	9.6
IL-4	2.4	8.4
IL-6	2.2	8.8
IL-8	3.2	8.4
IL-9	6.2	10.3
IL-10	3.6	11.2
IL-12 p70	2.6	9.8
IL-13	2.5	8.3
IL-15	3.2	11.2
IL-17A	2.5	9.8
IL-33	2.9	9.7
IP-10	3.3	6.6
MCP-1	1.8	6.3
MIP-1 alpha	2.9	5.7
MIP-1 beta	1.7	7.9
PD-L1/B7-H1	2.7	8.3
TNF-alpha	3.0	10.3

Accuracy

	Linearity		Spiked Recovery
Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
Analyte	N	Average %	N	Average %
CD40 Ligand	4	131	4	75
GM-CSF	4	101	4	98
Granzyme B	4	107	4	96
IFN-alpha 2	4	106	4	87
IFN-gamma	4	127	4	73
IL-1 alpha	4	143	3	74
IL-1 beta	4	106	4	94
IL-1ra*	4	126	HE
IL-2	4	115	4	88
IL-4	4	141	4	58
IL-6	4	126	4	78
IL-8	4	118	4	78
IL-9	4	111	4	96
IL-10	4	117	4	90
IL-12 p70	4	111	4	87
IL-13	4	118	4	89
IL-15	4	103	4	76
IL-17A	4	128	4	72
IL-33	4	103	4	87
IP-10*	4	124	4	72
MCP-1*	4	80	HE
MIP-1 alpha	4	95	4	111
MIP-1 beta	4	109	4	95
PD-L1/B7-H1	4	106	4	96
TNF-alpha	4	134	4	71
*Natural linearity HE = Spiked recovery was not observed due to natural linewarity in high endogenous samples.

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.