Human IL-7R alpha/CD127 APC-conjugated Antibody Summary
Extracellular domain
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 APC-conjugated Monoclonal Antibody (Catalog # FAB306A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-7R alpha/CD127
Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).
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- Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
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- Jahn, T. et al. (2007) Blood 110:1840.
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Product Datasheets
Citations for Human IL-7R alpha/CD127 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Impact on early outcomes and immune reconstitution of high-dose post-transplant cyclophosphamide vs anti-thymocyte globulin after reduced intensity conditioning peripheral blood stem cell allogeneic transplantation
Authors: C Retière, C Willem, T Guillaume, H Vié, L Gautreau-R, E Scotet, X Saulquin, K Gagne, MC Béné, BM Imbert, B Clemenceau, P Peterlin, A Garnier, P Chevallier
Oncotarget, 2018-01-27;9(14):11451-11464.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
The Proteomic Landscape of Human Ex Vivo Regulatory and Conventional T Cells Reveals Specific Metabolic Requirements
Authors: Claudio Procaccini, Fortunata Carbone, Dario Di Silvestre, Francesca Brambilla, Veronica De Rosa, Mario Galgani et al.
Immunity
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Regulatory T cell proliferative potential is impaired in human autoimmune disease.
Authors: Carbone F, De Rosa V, Carrieri P, Montella S, Bruzzese D, Porcellini A, Procaccini C, La Cava A, Matarese G
Nat Med, 2013-12-08;20(1):69-74.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Biomarkers on melanoma patient T cells associated with ipilimumab treatment.
Authors: Wang W, Yu D, Sarnaik A, Yu B, Hall M, Morelli D, Zhang Y, Zhao X, Weber J
J Transl Med, 2012-07-12;10(0):146.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Transient regulatory T-cells: a state attained by all activated human T-cells.
Authors: Pillai V, Ortega SB, Wang CK, Karandikar NJ
Clin. Immunol., 2006-12-19;123(1):18-29.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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