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Human IL-7R alpha/CD127 Antibody

Catalog #: AF-306-PB
7 Citations 1 Review Datasheet / COA / SDS
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AF-306-PB
AF-306-SP
Detection of Human IL-7R alpha/CD127 by Flow Cytometry
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Citations (7)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-7R alpha/CD127 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-7 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant rat IL-7 R alpha and less than 5% cross-reactivity with recombinant mouse IL‑7 R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha /CD127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (Catalog # 306-IR)
Flow Cytometry
0.25 µg/106 cells
Human peripheral blood CD4+ lymphocytes
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Human IL-7R alpha/CD127 by Flow Cytometry View Larger

Detection of Human IL-7R alpha/CD127 by Flow Cytometry IL-7 response of CD4+ T cells from tuberculosis patients and healthy contacts.(a) IL-7-induced (10 ng/ml) STAT5 phosphorylation of CD4+ T cells from tuberculosis patients (n = 22) and healthy contacts (n = 24) measured by flow cytometry. The STAT5 phosphorylation level of non-stimulated cells has been subtracted of all values. (b) IFN gamma /CD40L-expressing CD4+ T cells after PPD re-stimulation in the presence or absence of IL-7 detected by flow cytometry. Non-stimulated values with or without IL-7 have been subtracted. (c) Induction of IFN gamma /CD40L-expressing CD4+ T cells by IL-7 and PPD stimulation. Absolute differences as compared to PPD alone are shown. (d) Plasma IL-6 levels of tuberculosis patients (n = 20) and healthy contacts (n = 24). An arbitrary threshold indicated by a dotted line was set to define tuberculosis patients with high (IL-6high) and low (IL-6low) concentrations of plasma IL-6. (e) STAT5 phosphorylation, and IL-7-induced PPD response (f) for tuberculosis patients with high or low plasma IL-6 level, as defined in (d). Median and interquartile range is depicted, and exact Mann-Whitney U test was applied for comparison of groups, whereas paired data was evaluated by Wilcoxon Signed-Rank test. Nominal p-values are indicated as * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.ppat.1006425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human IL-7R alpha/CD127 by Flow Cytometry View Larger

Detection of Human IL-7R alpha/CD127 by Flow Cytometry Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) analysed by flow cytometry. (a) Mean fluorescence intensity (MFI) analyses of IL-7R. (b) Proportions of IL-7Rlow-expressing CD4+ and CD8+ T cells from tuberculosis patients and healthy contacts. Median and interquartile range is depicted, and each symbol indicates mean values of duplicates from each individual donor. Exact Mann-Whitney U test used for comparison of groups. Nominal p-values are indicated as * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.ppat.1006425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human IL-7R alpha/CD127 by Flow Cytometry View Larger

Detection of Human IL-7R alpha/CD127 by Flow Cytometry Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) analysed by flow cytometry. (a) Mean fluorescence intensity (MFI) analyses of IL-7R. (b) Proportions of IL-7Rlow-expressing CD4+ and CD8+ T cells from tuberculosis patients and healthy contacts. Median and interquartile range is depicted, and each symbol indicates mean values of duplicates from each individual donor. Exact Mann-Whitney U test used for comparison of groups. Nominal p-values are indicated as * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.ppat.1006425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Human IL-7R alpha/CD127 by Flow Cytometry View Larger

Detection of Human IL-7R alpha/CD127 by Flow Cytometry Membrane-associated IL-7R expression on CD4+ and CD8+ T cells.Membrane-associated IL-7R expression of CD4+ (left graphs) and CD8+ (right graphs) T cells from tuberculosis patients (n = 17) and healthy contacts (n = 21) analysed by flow cytometry. (a) Mean fluorescence intensity (MFI) analyses of IL-7R. (b) Proportions of IL-7Rlow-expressing CD4+ and CD8+ T cells from tuberculosis patients and healthy contacts. Median and interquartile range is depicted, and each symbol indicates mean values of duplicates from each individual donor. Exact Mann-Whitney U test used for comparison of groups. Nominal p-values are indicated as * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.ppat.1006425), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References
  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.
Long Name
Interleukin 7 Receptor alpha
Entrez Gene IDs
3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

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Citations for Human IL-7R alpha/CD127 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Cytokine-induced transient monocyte IL-7Ra expression and the serum milieu in tuberculosis
    Authors: JD Harelimana, HS Ahor, B Benner, S Hellmuth, E Adankwah, D Minadzi, W Aniagyei, MNK Lamptey, J Arthur, A Yeboah, MK Abass, LB Debrah, DO Owusu, E Mayatepek, J Seyfarth, RO Phillips, M Jacobsen
    European Journal of Immunology, 2022-03-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
  2. Increased soluble IL-7 receptor concentrations associate with improved IL-7 therapy outcomes in SIV-infected ART-treated Rhesus macaques
    Authors: AK Steele, L Carrasco-M, DL Sodora, AM Crawley
    PLoS ONE, 2017-12-19;12(12):e0188427.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
    Applications: Luminex Development
  3. Aberrant plasma IL-7 and soluble IL-7 receptor levels indicate impaired T-cell response to IL-7 in human tuberculosis
    Authors: C Lundtoft, A Afum-Adjei, J Rimpler, K Harling, N Nausch, M Kohns, E Adankwah, F Lang, L Olbrich, E Mayatepek, E Owusu-Dabo, M Jacobsen
    PLoS Pathog., 2017-06-05;13(6):e1006425.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. DNA Methylation Regulates the Differential Expression of CX3CR1 on Human IL-7Ralphalow and IL-7Ralphahigh Effector Memory CD8+ T Cells with Distinct Migratory Capacities to the Fractalkine.
    Authors: Shin M, You S, Kang Y, Lee N, Yoo S, Park K, Kang K, Kim S, Mohanty S, Shaw A, Montgomery R, Hwang D, Kang I
    J Immunol, 2015-08-14;195(6):2861-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line.
    Authors: Takao T, Asanoma K, Kato K
    PLoS ONE, 2011-07-07;6(7):e21990.
    Species: Human
    Sample Types: Whole Cells
    Applications: Cell Selection
  6. Soluble HIV Tat protein removes the IL-7 receptor alpha-chain from the surface of resting CD8 T cells and targets it for degradation.
    Authors: Faller EM, Sugden SM, McVey MJ
    J. Immunol., 2010-07-26;185(5):2854-66.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  7. A crucial role for Kupffer cell-derived galectin-9 in regulation of T cell immunity in hepatitis C infection.
    Authors: Mengshol JA, Golden-Mason L, Arikawa T
    PLoS ONE, 2010-03-04;5(3):e9504.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human IL-7 R alpha /CD127 Antibody
By Sarah Jaslow on 03/17/2017
Application: WB Sample Tested: HeLa cells Species: Human
Western Blot Human IL-7 R alpha /CD127 Antibody AF-306-PB