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Human IL-7R alpha/CD127 Antibody

Catalog #: MAB306
18 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB306-SP
MAB306-100
MAB306-500
Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (18)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-7R alpha/CD127 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-7 R alpha in Western blots. In Western blots, approximately 5% cross-reactivity was observed with recombinant human
(rh) IL‑10 R, rhIL‑2 R beta, rhIL‑5 R alpha, and rhIL‑6 R.
Source
Monoclonal Mouse IgG1 Clone # 40131
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL-7 R alpha
Extracellular domain
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑7 R alpha /CD127 Fc Chimera (Catalog # 306-IR)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IL-7 Ra/CD127 antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of IL‑7 R alpha /CD127 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e PE-conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human IL-7 Ra/CD127 Monoclonal Antibody (Catalog # MAB306) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Western Blot Detection of Human IL-7R alpha/CD127 by Western Blot View Larger

Detection of Human IL-7R alpha/CD127 by Western Blot Steroid resistance induced by wild-type or mutant IL7R signaling molecules is associated with activation of MEK-ERK and/or AKT.(A) Western blot results for total and/or phosphorylated levels of IL7R signaling molecules following doxycycline induction (+Dox) of wild-type or mutant forms of IL7R, JAK1, JAK3, NRAS, or AKT molecules in SUPT1 cells. The steroid-sensitive and -resistant panels are indicated. Cellular lysate of parental SUPT1 cells was used as a control. (B–G) beta -actin-normalized protein concentrations for (B) NR3C1, (C) pMEK, (D) pERK, (E) pAKT, (F) pGSK3B, and (G) BCLXL in doxycycline-induced steroid-sensitive and -resistant SUPT1 lines. Significance levels were calculated using the Mann-Whitney U test. In (E), phospho-AKT levels are shown for all lines except for lines induced to express construct-driven AKT and AKTE17K. (H) Schematic overview of crosstalk between the proapoptotic NR3C1 response following steroid exposure and activation of MEK-ERK and AKT pathways downstream of IL7R signaling mutations. Green vectors indicate molecules that drive a proapoptotic, steroid-sensitive response, whereas red vectors indicate molecules that drive an antiapoptotic, steroid-resistant response. See also S7 Fig. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27997540), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7R alpha/CD127

Interleukin 7 Receptor alpha (IL-7 R alpha ), also known as CD127, is a 75 kDa hematopoietin receptor superfamily member that plays an important role in lymphocyte differentiation, proliferation, and survival (1, 2). Mature human IL-7 R alpha consists of a 219 amino acid (aa) extracellular domain (ECD) with one fibronectin type-III domain and a WSXWS motif, a 25 aa transmembrane segment, and a 195 aa cytoplasmic domain (3). Alternate splicing of human IL-7 R alpha generates a secreted soluble form of the receptor (3). Within the ECD, human IL‑7 R alpha shares 67% aa sequence identity with mouse and rat IL-7 R alpha. IL-7 R alpha associates with the common gamma chain ( gamma c) to form the functional high affinity IL-7 receptor complex (4). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. Human and mouse IL‑7 show cross-species activity through the IL-7 receptor (3, 5). IL-7 R alpha is expressed on double negative (CD4-/CD8-) and CD4+ or CD8+ single positive T cells as well as on CD8+ memory T cells and their precursors (6, 7). It is expressed early in B cell development, prior to the appearance of surface IgM (6). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development (8). In human, by contrast, it is required for T cell but not for B cell development (9). IL-7 induces the downregulation and shedding of cell surface IL‑7 R alpha (10). IL-7 R alpha additionally associates with TSLP R to form the functional receptor for thymic stromal lymphopoietin (11, 12). TSLP indirectly regulates T cell development by modulating dendritic cell activation (2, 13). Knockout of TSLP R in mice provokes minor changes in B and T cell development compared to those seen with IL-7 R alpha deletion (8, 14). The complexity of IL-7 R alpha biology is suggested by the competition between IL-7 and TSLP for receptor binding and by the ability of IL-7 R alpha to form functional complexes with SCF R and HGF R (11, 12, 15, 16).

References
  1. Mazzucchelli, R. and S.K. Durum (2007) Nat. Rev. Immunol. 7:144.
  2. Liu, Y-J. et al. (2007) Annu. Rev. Immunol. 25:193.
  3. Goodwin, R.G. et al. (1990) Cell 60:941.
  4. Noguchi, M. et al. (1993) Science 262:1877.
  5. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  6. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90:9125.
  7. Kaech, S.M. et al. (2003) Nat. Immunol. 4:1191.
  8. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  9. Prieyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. USA 93:10348.
  10. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  11. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  12. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
  13. Reche, P.A. et al. (2001) J. Immunol. 167:336.
  14. Al-Shami, A. et al. (2004) J. Exp. Med. 200:159.
  15. Jahn, T. et al. (2007) Blood 110:1840.
  16. Lai, L. et al. (2006) Blood 107:1776.
Long Name
Interleukin 7 Receptor alpha
Entrez Gene IDs
3575 (Human); 16197 (Mouse); 294797 (Rat); 102143679 (Cynomolgus Monkey)
Alternate Names
CD127 antigen; CD127; CD127ILRA; CDW127; IL-7 R alpha; IL-7 receptor subunit alpha; IL7R alpha; IL-7R alpha; IL-7R subunit alpha; IL7R; IL7RA; IL-7Ra; IL-7R-alpha; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; interleukin 7 receptor; interleukin-7 receptor subunit alpha

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Citations for Human IL-7R alpha/CD127 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. MEK and PI3K-AKT inhibitors synergistically block activated IL7 receptor signaling in T-cell acute lymphoblastic leukemia
    Authors: K Canté-Barrett, J A P Spijkers-Hagelstein, J G C A M Buijs-Gladdines, J C M Uitdehaag, W K Smits, J van der Zwet et al.
    Leukemia
  2. Antisense modulation of IL7R splicing to control sIL7R expression in human CD4+ T cells
    Authors: Gaddiel Galarza-Muñoz, Debbie Kennedy-Boone, Geraldine Schott, Shelton S. Bradrick, Mariano A. Garcia-Blanco
    RNA
  3. Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood
    Authors: Natalia Sigal, Holden T. Maecker
    Methods in Molecular Biology
  4. Interleukin-7 dependent non-classical monocytes and CD40 expression are affected in children with type 1 diabetes
    Authors: P Hehenkamp, M Hoffmann, S Kummer, C Reinauer, C Döing, K Förtsch, J Enczmann, V Balz, E Mayatepek, T Meissner, M Jacobsen, J Seyfarth
    European Journal of Immunology, 2021-11-01;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  5. Synergism between IL7R and CXCR4 drives BCR-ABL induced transformation in Philadelphia chromosome-positive acute lymphoblastic leukemia
    Authors: H Abdelrasou, A Vadakumche, M Werner, L Lenk, A Khadour, M Young, O El Ayoubi, F Vogiatzi, M Krämer, V Schmid, Z Chen, Y Yousafzai, G Cario, M Schrappe, M Müschen, C Halsey, MA Mulaw, DM Schewe, E Hobeika, A Alsadeq, H Jumaa
    Nat Commun, 2020-06-24;11(1):3194.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. IL-7 induces sCD127 release and mCD127 downregulation in human CD8+ T cells by distinct yet overlapping mechanisms, both of which are impaired in HIV infection
    Authors: SC Côté, SCB Schinkel, TK Berthoud, PO Barros, M Sanchez-Vi, AM Davidson, AM Crawley, JB Angel
    Eur. J. Immunol., 2020-05-27;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  7. Context-specific regulation of surface and soluble IL7R expression by an autoimmune risk allele
    Authors: H Al-Mossawi, N Yager, CA Taylor, E Lau, S Danielli, J de Wit, J Gilchrist, I Nassiri, EA Mahe, W Lee, L Rizvi, S Makino, J Cheeseman, M Neville, JC Knight, P Bowness, BP Fairfax
    Nat Commun, 2019-10-08;10(1):4575.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  8. Macrophages are the primary effector cells in IL-7-induced arthritis
    Authors: SJ Kim, HJ Chang, MV Volin, S Umar, K Van Raemdo, A Chevalier, K Palasiewic, JW Christman, S Volkov, S Arami, M Maz, A Mehta, RK Zomorrodi, DA Fox, N Sweiss, S Shahrara
    Cell. Mol. Immunol., 2019-06-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. CD56(bright) NK IL-7R? expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections
    Authors: CJ Judge, L Kostadinov, KE Sherman, AA Butt, Y Falck-Ytte, NT Funderburg, AL Landay, MM Lederman, SF Sieg, JK Sandberg, DD Anthony
    J. Leukoc. Biol., 2017-04-11;0(0):.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  10. IL-7 Receptor Mutations and Steroid Resistance in Pediatric T cell Acute Lymphoblastic Leukemia: A Genome Sequencing Study
    PLoS Med, 2016-12-20;13(12):e1002200.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Regulatory T cells inhibit CD34+ cell differentiation into NK cells by blocking their proliferation.
    Authors: Isabela Pedroza-Pa, Divya Shah, Anna Domogala, Martha Luevano, Michael Blundell, Nicola Jackson, Adrian Thrasher, Alejandro Madrigal, Aurore Saudemont
    Scientific Reports, 2016-02-26;0(0):2045-2322.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. The clinical correlation of regulatory T cells and cyclic adenosine monophosphate in enterovirus 71 infection.
    Authors: Wang, Shih-Min, Chen, I-Chun, Liao, Yu-Ting, Liu, Ching-Ch
    PLoS ONE, 2014-07-10;9(7):e102025.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Decreased systemic IL-7 and soluble IL-7Ralpha in multiple sclerosis patients.
    Genes Immun, 2012-08-23;13(7):587-92.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  14. Interleukin-7 compartmentalizes its receptor signaling complex to initiate CD4 T lymphocyte response.
    Authors: Rose T, Pillet AH, Lavergne V, Tamarit B, Lenormand P, Rousselle JC, Namane A, Theze J
    J. Biol. Chem., 2010-02-18;285(20):14898-908.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  15. Functionally significant differences in expression of disease-associated IL-7 receptor alpha haplotypes in CD4 T cells and dendritic cells.
    Authors: Hoe E, McKay FC, Schibeci SD, Gandhi K, Heard RN, Stewart GJ, Booth DR
    J. Immunol., 2010-01-22;184(5):2512-7.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  16. Identification and biochemical characterization of human plasma soluble IL-7R: lower concentrations in HIV-1-infected patients.
    Authors: Rose T, Lambotte O, Pallier C, Delfraissy JF, Colle JH
    J. Immunol., 2009-06-15;182(12):7389-97.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development, Immunoprecipitation
  17. Establishment and characterization of SRIK-NKL: a novel CD8+ natural killer/T cell line derived from a patient with leukemic phase of acute lymphoblastic lymphoma.
    Authors: Srivastava BI, Srivastava MD
    Leuk. Res., 2005-07-01;29(7):771-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Translation of IRF-1 Restricts Hepatic Interleukin-7 Production to Types I and II Interferons: Implications for Hepatic Immunity
    Authors: Sabrina Rueschenbaum, Chengcong Cai, Matthias Schmidt, Katharina Schwarzkopf, Ulf Dittmer, Stefan Zeuzem et al.
    Frontiers in Immunology

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Human IL-7R alpha/CD127 Antibody
By Anonymous on 08/30/2021
Application: WB Sample Tested: Adult kidney Species: Human