Human IL-6R alpha Fluorescein-conjugated Antibody

Catalog #: FAB227F
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB227F-100
FAB227F-025

Detection of IL‑6 R alpha in Human Blood Lymphocytes by Flow Cytometry.

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All IL-6R alpha Antibodies

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Citations (2)

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Human IL-6R alpha Fluorescein-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-6 R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.

Source

Monoclonal Mouse IgG₁ Clone # 17506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑6 R alpha
Leu20-Asp339
Accession # P08887

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

- Human peripheral blood lymphocytes

- U937 human histiocytic lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑6 R alpha in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3e APC-conjugated Monoclonal Antibody (FAB100A) and either (A) Mouse Anti-Human IL-6 Ra Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB227F) or (B) Mouse IgG₁Fluorescein Isotype Control (IC002F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of IL-6R alpha in U937 cells by Flow Cytometry U937 cells were stained with Mouse Anti-Human IL-6R alpha Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB227F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

References

Yamasaki et al. (1988) Science 241:825.
Baumann et al. (1990) J. Biol. Chem. 265:19853.
Hibi et al. (1990) Cell 63:1149.
Schooltink et al. (1991) Eur. J. Biochem. 277:659.
Novick et al. (1989) J. Exp. Med. 170:1409.
Yasukawa et al. (1990) J. Biochem. 108:673.
Saito et al. (1991) J. Immunology 147:168.

Long Name

Interleukin 6 Receptor alpha

Entrez Gene IDs

3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)

Alternate Names

CD126; gp80; IL-6 R alpha; IL6Q; IL6R alpha; IL-6R alpha; IL6R; IL-6R-1; IL6RA; IL-6Ra; IL6RQ; interleukin 6 receptor

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Citations for Human IL-6R alpha Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Plasminogen Activator Inhibitor-1 Promotes the Recruitment and Polarization of Macrophages in Cancer
Authors: MH Kubala, V Punj, VR Placencio-, H Fang, GE Fernandez, R Sposto, YA DeClerck
Cell Rep, 2018-11-20;25(8):2177-2191.e7.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
IL-27 mediates HLA class I up-regulation, which can be inhibited by the IL-6 pathway, in HLA-deficient Small Cell Lung Cancer cells
Authors: Grazia Carbotti, Amin Reza Nikpoor, Paola Vacca, Rosaria Gangemi, Chiara Giordano, Francesco Campelli et al.
Journal of Experimental & Clinical Cancer Research