Human IL-6/IL-6R alpha Complex DuoSet ELISA

Catalog # Availability Size / Price Qty
DY8139-05
Ancillary Products Available
Human IL-6 / IL-6 R alpha Complex ELISA Standard Curve
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (2)

Human IL-6/IL-6R alpha Complex DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
78.1 - 5,000 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human IL-6 / IL-6 R alpha Complex. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human IL-6 / IL-6 R alpha Complex ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-6/IL-6R alpha Complex

IL-6 (Interleukin-6) is a pleiotropic cytokine that acts in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 signals through a receptor complex of IL-6 R alpha and gp130. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha.

Long Name:
Interleukin 6/Interleukin 6 Receptor alpha Complex
Entrez Gene IDs:
3570 (Human); 16194 (Mouse); 24499 (Rat)
Alternate Names:
IL-6/IL-6R alpha Complex

Citations for Human IL-6/IL-6R alpha Complex DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Activation of STAT3 signaling is mediated by TFF1 silencing in gastric neoplasia
    Authors: M Soutto, Z Chen, AA Bhat, L Wang, S Zhu, A Gomaa, A Bates, NS Bhat, D Peng, A Belkhiri, MB Piazuelo, MK Washington, XC Steven, R Peek, W El-Rifai
    Nat Commun, 2019-07-10;10(1):3039.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. The balance of�Interleukin (IL)-6, IL-6:soluble IL-6 receptor (IL-6R) and IL-6:sIL-6R:sgp130 complexes allows simultaneous classic and trans-signaling
    Authors: P Baran, S Hansen, GH Waetzig, M Akbarzadeh, L Lamertz, HJ Huber, MR Ahmadian, JM Moll, J Scheller
    J. Biol. Chem., 2018-03-20;0(0):.

FAQs

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Reviews for Human IL-6/IL-6R alpha Complex DuoSet ELISA

Average Rating: 4 (Based on 2 Reviews)

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Human IL-6/IL-6R alpha Complex DuoSet ELISA
By Anonymous on 04/02/2020
Sample Tested: Serum and Plasma

We used this kit for the quantification of IL-6R in human serum and plasma. Works very well and well-described protocol.


Human IL-6/IL-6 R alpha Complex DuoSet ELISA
By Anonymous on 11/01/2016
Sample Tested: Serum

Works as expected