Human IL-3R alpha/CD123 Antibody Summary
Lys20-Arg305, predicted
Accession # P26951
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑3 R alpha /CD123 in Human Peripheral Blood Lymphocytes. IL‑3 R alpha /CD123 was detect-ed in immersion fixed human peripheral blood lymphocytes using 2 µg/mL Mouse Anti-Human IL‑3 R alpha /CD123 Monoclonal Antibody (Catalog # MAB301) for 3 hours at room temperature. Cells were stained (red) and counter-stained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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IL‑3 R alpha /CD123 in Human Tonsil. IL-3 Ra/CD123 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Cell Proliferation Induced by IL‑3 and Neutralization by Human IL‑3 R alpha /CD123 Antibody. Recombinant Human IL-3 (203-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-3 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-3 Ra/CD123 Monoclonal Antibody (Catalog # MAB301). The ND50 is typically 0.6-1.2 µg/mL.
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Detection of IL-3R alpha/CD123 in THP-1 cells by Flow Cytometry. THP-1 cells were stained with Mouse Anti-Human IL-3R alpha/CD123 Monoclonal Antibody (Catalog # MAB301, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-3R alpha
IL-3 is a pleiotropic cytokine that can stimulate proliferation and differentiation of pluripotent hematopoietic stem cells as well as various lineage committed progenitors (1, 2). IL-3 exerts its activity through binding to a specific cell surface receptor known as IL-3 R. IL-3 R is a heterodimeric structure composed of a 70 kDa IL-3 R alpha subunit (CD123) and a 120-140 kDa IL-3 R beta subunit (CD131) (3, 4). IL-3 R alpha binds IL-3 with relatively low affinity. In the presence of IL-3 R beta, however, IL-3 R alpha has a much higher affinity for IL-3. It is not clear how signal transduction occurs following IL-3 binding. The IL-3 R alpha chain has a very short intracellular domain while the IL‑3 R beta chain has a very large cytoplasmic domain. The IL‑3 R beta chain is also shared by the receptors for IL-5 and GM-CSF. Cells known to express IL-3 receptors include hematopoietic progenitors, epithelial cells, double negative T cells, mast cells, basophils and blood monocytes (5).
- Moore, M.A.S. et al. (1991) Blood 72:944.
- Warren, D.J. et al. (1988) J. Immunol. 140:94.
- Plant M. et al. (1989) Nature 339:150.
- Budel, L.M. et al. (1990) Blood 75:1439.
- Schrader, J.W. et al. (1988) In Interleukin-3: The Panspecific hemopoietin (ed. J.W. Schrader), Academic Press, San Diego, CA.
Product Datasheets
Citations for Human IL-3R alpha/CD123 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
10
Citations: Showing 1 - 10
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Activated Stat5 trafficking Via Endothelial Cell-derived Extracellular Vesicles Controls IL-3 Pro-angiogenic Paracrine Action
Sci Rep, 2016-05-09;6(0):25689.
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A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes
Authors: M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter
Elife, 2016-03-23;5(0):.
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Interleukin-3-Receptor-alpha in Triple-Negative Breast Cancer (TNBC): An Additional Novel Biomarker of TNBC Aggressiveness and a Therapeutic Target
Authors: M Koni, I Castellano, E Venturelli, A Sarcinella, T Lopatina, C Grange, M Cedrino, S Femminò, P Cossu-Rocc, S Orrù, F D'Ascenzo, I Cotellessa, C Tampieri, C Debernardi, G Cugliari, G Matullo, G Camussi, MR De Miglio, MF Brizzi
Cancers, 2022-08-13;14(16):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Targeting IL-3R&alpha on tumor-derived endothelial cells blunts metastatic spread of triple-negative breast cancer via extracellular vesicle reprogramming
Authors: T Lopatina, C Grange, C Cavallari, V Navarro-Ta, G Lombardo, A Rosso, M Cedrino, MAC Pomatto, M Koni, F Veneziano, I Castellano, G Camussi, MF Brizzi
Oncogenesis, 2020-10-10;9(10):90.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Myelodysplastic syndrome progression to acute myeloid leukemia at the stem cell level
Authors: J Chen, YR Kao, D Sun, TI Todorova, D Reynolds, SR Narayanaga, C Montagna, B Will, A Verma, AU Steidl
Nat. Med., 2018-12-03;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
A dual role for the N-terminal domain of the IL-3 receptor in cell signalling
Authors: SE Broughton, TR Hercus, TL Nero, WL Kan, EF Barry, M Dottore, KS Cheung Tun, CJ Morton, U Dhagat, MP Hardy, NJ Wilson, MT Downton, C Schieber, TP Hughes, AF Lopez, MW Parker
Nat Commun, 2018-01-26;9(1):386.
Applications: Flow Cytometry -
Interleukin-3 enhances the migration of human mesenchymal stem cells by regulating expression of CXCR4
Authors: A Barhanpurk, ST Mhaske, ST Pote, K Singh, MR Wani
Stem Cell Res Ther, 2017-07-14;8(1):168.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human Basophils Modulate Plasma Cell Differentiation and Maturation
Authors: Almut Meyer-Bahl
J. Immunol., 2016-11-16;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Graves' disease is associated with a defective expression of the immune regulatory molecule galectin-9 in antigen-presenting dendritic cells.
Authors: Leskela S, Serrano A, de la Fuente H, Rodriguez-Munoz A, Ramos-Levi A, Sampedro-Nunez M, Sanchez-Madrid F, Gonzalez-Amaro R, Marazuela M
PLoS ONE, 2015-04-16;10(4):e0123938.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr -
Human T-cell leukemia virus type 2 induces survival and proliferation of CD34(+) TF-1 cells through activation of STAT1 and STAT5 by secretion of interferon-gamma and granulocyte macrophage-colony-stimulating factor.
Authors: Bovolenta C, Pilotti E, Mauri M, Turci M, Ciancianaini P, Fisicaro P, Bertazzoni U, Poli G, Casoli C
Blood, 2002-01-01;99(1):224-31.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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