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Human IL-33 ELISA Kit - Quantikine

Catalog #: D3300B
31 Citations Datasheet / COA / SDS
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QC244: Quantikine Immunoassay Control Group 244 for Human IL-33
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Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve
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Citations (31)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-33 Quantikine ELISA Kit Summary

Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (10 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
1.51 pg/mL
Assay Range
3.1 - 200 pg/mL (Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-33.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-33 Immunoassay is a 4.0 hour solid phase ELISA designed to measure IL-33 in cell culture supernates, cell lysates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant human IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-33. Results obtained using natural human IL-33 showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-33.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision over one lot of kit components. Assays were performed by at least three technicians

Cell Culture Supernates, Cell Lysates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 31 59.8 119 31 59.5 117
Standard Deviation 1.15 2.72 6.99 1.35 2.6 6.98
CV% 3.7 4.5 5.9 4.4 4.4 6

Recovery

The recovery of human IL-33 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 97 88-107
Cell Lysis Buffer (n=4) 103 86-123
EDTA Plasma (n=4) 91 83-103
Extraction Buffer (1X) (n=2) 103 81-115
Heparin Plasma (n=4) 89 85-95
Serum (n=4) 89 85-96

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IL-33 ELISA Linearity
Human IL-33 ELISA Linearity

Scientific Data

N/A Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve View Larger

Human IL-33 ELISA Cell Culture Supernate/Cell Lysate Standard Curve

N/A Human IL-33 ELISA Standard Curve View Larger

Human IL-33 ELISA Standard Curve

N/A Human IL-33 ELISA Serum/Plasma Standard Curve View Larger

Human IL-33 ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-33

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

Long Name:
Interleukin 33
Entrez Gene IDs:
90865 (Human); 77125 (Mouse)
Alternate Names:
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human IL-33 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Endothelial CD34 expression and regulation of immune cell response in-vitro
    Authors: Arakelian, L;Lion, J;Churlaud, G;Bargui, R;Thierry, B;Mutabazi, E;Bruneval, P;Alberdi, AJ;Doliger, C;Veyssiere, M;Larghero, J;Mooney, N;
    Scientific reports
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Airway epithelium IgE-Fc?RI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma
    Authors: Weng, CM;Lee, MJ;Chao, W;Lin, YR;Chou, CJ;Chen, MC;Chou, CL;Lin Tsai, I;Lin, CH;Fan Chung, K;Kuo, HP;
    Mucosal immunology
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Serum Levels of Selected IL-1 Family Cytokines in Patients with Morphea
    Authors: P Szczepanik, M Michalska-, M Kowal, D Krasowska
    Journal of Clinical Medicine, 2022-10-28;11(21):.
    Species: Human
    Sample Types: Serum
  4. Allergen protease-activated stress granule assembly and gasdermin D fragmentation control interleukin-33 secretion
    Authors: W Chen, S Chen, C Yan, Y Zhang, R Zhang, M Chen, S Zhong, W Fan, S Zhu, D Zhang, X Lu, J Zhang, Y Huang, L Zhu, X Li, D Lv, Y Fu, H Iv, Z Ling, L Ma, H Jiang, G Long, J Zhu, D Wu, B Wu, B Sun
    Nature Immunology, 2022-07-06;23(7):1021-1030.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. The Merkel Cell Polyomavirus T-Antigens and IL-33/ST2-IL1RAcP Axis: Possible Role in Merkel Cell Carcinoma
    Authors: K Rasheed, U Moens, B Policastro, JI Johnsen, V Koljonen, H Sihto, WO Lui, B Sveinbjørn
    International Journal of Molecular Sciences, 2022-03-28;23(7):.
    Species: Human
    Sample Types: Plasma
  6. Development of an ultra-sensitive human IL-33 biomarker assay for age-related macular degeneration and asthma drug development
    Authors: E Mai, J Chan, L Goon, BK Ego, J Bevers, T Wong, M Wong, R Corpuz, H Xi, J Wu, K Schneider, D Seshasayee, M Grimbaldes, G Nakamura, VB Indjeian, M van Looker, KM Loyet, L Comps-Agra
    Journal of Translational Medicine, 2021-12-20;19(1):517.
    Species: Human
    Sample Types: Vitreous Humor
  7. Interleukin-33 as an early predictor of cetuximab treatment efficacy in patients with colorectal cancer
    Authors: X Zhang, K Bi, X Tu, Q Zhang, Q Cao, Y Liang, P Zeng, L Wang, T Liu, W Fang, H Diao
    Cancer Medicine, 2021-10-19;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Interleukin-31 promotes fibrosis and T helper 2 polarization in systemic sclerosis
    Authors: A Kuzumi, A Yoshizaki, KM Matsuda, H Kotani, Y Norimatsu, M Fukayama, S Ebata, T Fukasawa, A Yoshizaki-, Y Asano, K Morikawa, Y Kazoe, K Mawatari, T Kitamori, S Sato
    Nature Communications, 2021-10-12;12(1):5947.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates, Serum, Tissue Homogenates
  9. Respiratory Nasal Mucosa in Chronic Rhinosinusitis with Nasal Polyps versus COVID-19: Histopathology, Electron Microscopy Analysis and Assessing of Tissue Interleukin-33
    Authors: II Jeican, D Gheban, L Barbu-Tudo, P Ini?ca, C Albu, M Ilie?, S Albu, ML Vic?, HV Matei, S Tripon, M Laz?r, M Alua?, CV Siserman, M Muntean, V Trombitas, CA Iuga, I Opincariu, LM Junie
    Journal of Clinical Medicine, 2021-09-12;10(18):.
    Species: Human
    Sample Types: Tissue Homogenates
  10. LYSMD3: A mammalian pattern recognition receptor for chitin
    Authors: X He, BA Howard, Y Liu, AK Neumann, L Li, N Menon, T Roach, SD Kale, DC Samuels, H Li, T Kite, H Kita, TY Hu, M Luo, CN Jones, UJ Okaa, DL Squillace, BS Klein, CB Lawrence
    Cell Reports, 2021-07-20;36(3):109392.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. Plasma Interleukin-33 level in relapsing-remitting multiple sclerosis. Is it negatively correlated with central nervous system lesions in patients with mild disability?
    Authors: H Mado, M Adamczyk-S, W Bartman, K Wierzbicki, B Tadeusiak, P Sowa
    Clinical neurology and neurosurgery, 2021-05-20;206(0):106700.
    Species: Human
    Sample Types: Plasma
  12. COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice
    Authors: W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles
    J. Immunol., 2020-07-20;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Clinical characteristics of patients with not well-controlled severe asthma in Japan: Analysis of the Keio Severe Asthma Research Program in Japanese population (KEIO-SARP) registry
    Authors: T Tanosaki, H Kabata, M Matsusaka, J Miyata, K Masaki, T Mochimaru, S Okuzumi, M Kuwae, R Watanabe, Y Suzuki, K Sayama, K Izuhara, K Asano, K Fukunaga
    Allergol Int, 2020-07-08;0(0):.
    Species: Human
    Sample Types: Serum
  14. IL-33 and its decoy sST2 in patients with Alzheimer's disease and mild cognitive impairment
    Authors: M Saresella, I Marventano, F Piancone, F La Rosa, D Galimberti, C Fenoglio, E Scarpini, M Clerici
    J Neuroinflammation, 2020-06-06;17(1):174.
    Species: Human
    Sample Types: CSF
  15. High plasma levels of pro-inflammatory factors interleukin-17 and interleukin-23 are associated with poor outcome of cardiac-arrest patients: a single center experience
    Authors: YG Zhuang, YZ Chen, SQ Zhou, H Peng, YQ Chen, DJ Li
    BMC Cardiovasc Disord, 2020-04-15;20(1):170.
    Species: Human
    Sample Types: Plasma
  16. Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris
    Authors: P Borsky, Z Fiala, C Andrys, M Beranek, K Hamakova, A Malkova, T Svadlakova, J Krejsek, V Palicka, L Borska, V Rehacek
    Mediators Inflamm., 2020-04-15;2020(0):8465083.
    Species: Human
    Sample Types: Serum
  17. Serum IL-1 β can be a biomarker in children with severe persistent allergic rhinitis
    Authors: MW Han, SH Kim, I Oh, YH Kim, J Lee
    Allergy Asthma Clin Immunol, 2019-09-18;15(0):58.
    Species: Human
    Sample Types: Serum
  18. Diagnostic role of interleukin -33 in the differentiation of pleural effusions especially tuberculous and malignant effusions
    Authors: AH Al-Aarag, MH Kamel, ER Abdelgawad, SM Abo-Yousse, HH Moussa, ME Elnaggar, RM Hendy, KA Diab
    BMC Pulm Med, 2019-06-25;19(1):114.
    Species: Human
    Sample Types: Serum
  19. Serum soluble ST2 as a marker of renal scar in pediatric upper urinary tract infection
    Authors: N Ohta, H Yasudo, M Mizutani, T Matsushige, R Fukano, S Kittaka, K Maehara, K Ichihara, S Ohga, S Hasegawa
    Cytokine, 2019-05-29;120(0):258-263.
    Species: Human
    Sample Types: Serum
  20. Serum sST2 levels predict severe exacerbation of asthma
    Authors: M Watanabe, K Nakamoto, T Inui, M Sada, K Honda, M Tamura, Y Ogawa, T Yokoyama, T Saraya, D Kurai, H Ishii, H Takizawa
    Respir. Res., 2018-09-03;19(1):169.
    Species: Human
    Sample Types: Serum
  21. In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity
    Authors: M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku
    Cell. Mol. Immunol., 2018-07-20;0(0):.
    Species: Human
    Sample Types: Serum
  22. Serum IL-33 level is associated with auto-antibodies but not with clinical response to biologic agents in rheumatoid arthritis
    Authors: E Rivière, J Sellam, J Pascaud, P Ravaud, JE Gottenberg, X Mariette
    Arthritis Res. Ther., 2018-06-08;20(1):122.
    Species: Human
    Sample Types: Serum
  23. Genetic variants in IL-33/ST2 pathway with the susceptibility to hepatocellular carcinoma in a Chinese population
    Authors: ZH Wei, YY Li, SQ Huang, ZQ Tan
    Cytokine, 2018-04-12;0(0):.
    Species: Human
    Sample Types: Plasma
  24. Immune and Imaging Correlates of Mild Cognitive Impairment Conversion to Alzheimer's Disease
    Authors: F La Rosa, M Saresella, F Baglio, F Piancone, I Marventano, E Calabrese, R Nemni, E Ripamonti, M Cabinio, M Clerici
    Sci Rep, 2017-12-01;7(1):16760.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Regulatory T cell frequency, but not plasma IL-33 levels, represents potential immunological biomarker to predict clinical response to intravenous immunoglobulin therapy
    Authors: MS Maddur, E Stephen-Vi, M Das, P Prakhar, VK Sharma, V Singh, M Rabin, J Trinath, KN Balaji, F Bolgert, JM Vallat, L Magy, SV Kaveri, J Bayry
    J Neuroinflammation, 2017-03-20;14(1):58.
    Species: Human
    Sample Types: Plasma
  26. Th1/Th17-Related Cytokines and Chemokines and Their Implications in the Pathogenesis of Pemphigus Vulgaris
    Authors: RP Timoteo, MV da Silva, CB Miguel, DA Silva, JD Catarino, V Rodrigues, H Sales-Camp, CJ Freire Oli
    Mediators Inflamm., 2017-02-22;2017(0):7151285.
    Species: Human
    Sample Types: Serum
  27. Upregulation of interleukin-33 and thymic stromal lymphopoietin levels in the lungs of idiopathic pulmonary fibrosis
    Authors: JU Lee, HS Chang, HJ Lee, CA Jung, DJ Bae, HJ Song, JS Park, ST Uh, YH Kim, KH Seo, CS Park
    BMC Pulm Med, 2017-02-15;17(1):39.
    Species: Human
    Sample Types: BALF
  28. Carotid plaque and bone density and microarchitecture in psoriatic arthritis: the correlation with soluble ST2
    Sci Rep, 2016-08-24;6(0):32116.
    Species: Human
    Sample Types: Plasma
  29. The challenge of measuring IL-33 in serum using commercial ELISA: lessons from asthma.
    Authors: Ketelaar M, Nawijn M, Shaw D, Koppelman G, Sayers I
    Clin Exp Allergy, 2016-06-01;46(6):884-7.
    Species: Human
    Sample Types: Serum
  30. IL-33 reflects dynamics of disease activity in patients with autoimmune hemolytic anemia by regulating autoantibody production.
    Authors: Bu X, Zhang T, Wang C, Ren T, Wen Z
    J Transl Med, 2015-12-16;13(0):381.
    Species: Human
    Sample Types: Serum
  31. High plasma levels of soluble ST2 but not its ligand IL-33 is associated with severe forms of pediatric dengue.
    Authors: Guerrero C, Arrieta A, Ramirez N, Rodriguez L, Vega R, Bosch I, Rodriguez J, Narvaez C, Salgado D
    Cytokine, 2013-01-26;61(3):766-71.
    Species: Human
    Sample Types: Plasma

FAQs

  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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