Human IL-31 Antibody

Catalog # Availability Size / Price Qty
MAB2824
MAB2824-SP
Product Details
FAQs
Supplemental Products
Reviews (1)

Human IL-31 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-31 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) IL-31 is observed.
Source
Monoclonal Mouse IgG1 Clone # 308227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-31
Ser24-Thr164
Accession # Q6EBC2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL‑31 (Catalog # 2824-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-31

Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein (1, 2). The mature region shows four alpha -helices which would be expected to show a typical up-up-down-down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region (1). IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL-31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ) (2‑6). In the complex, IL-31 directly binds to GPL, not OSM R (2, 3). IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway (2‑5). Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable (2, 3). The IL-31 receptor is constitutively expressed by keratinocytes and upregulated by IFN-gamma on monocytes (1). Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis (1, 7).

References
  1. Dillon, S.R. et al. (2004) Nat. Immunol. 5:752.
  2. Diveu, C. et al. (2004) Eur. Cytokine Netw. 15:291.
  3. Dreuw, A. et al. (2004) J. Biol. Chem. 279:36112.
  4. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  5. Ghilardi, N. et al. (2002) J. Biol. Chem 277:16831.
  6. Mosley, B. et al. (1996) J. Biol. Chem. 271:32635.
  7. Takaoka, A. et al. (2005) Eur. J. Pharmacol. 516:180.
Long Name
Interleukin 31
Entrez Gene IDs
386653 (Human); 76399 (Mouse)
Alternate Names
IL31; IL-31; IL-31interleukin-31; interleukin 31

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IL-31 Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Human IL-31 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human IL-31 Antibody
By Anonymous on 11/04/2017
Application: Flow Sample Tested: 2120Ep human embryonal carcinoma cell line Species: Human