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Human IL-23 ELISA Kit - Quantikine

Catalog #: D2300B
34 Citations 1 Review Datasheet / COA / SDS
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QC23: Quantikine Immunoassay Control Group 8
Human IL-23 ELISA Standard Curve
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Product Details
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Citations (34)
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Reviews (1)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-23 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
16.3 pg/mL
Assay Range
39.0 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-23. This assay does not recognize free p19 or p40 subunits.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human IL-23 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human IL-23 in cell culture supernates, serum, and plasma. It contains Sf 21-expressed recombinant human IL-23 and has been shown to accurately quantitate the recombinant factor. Results obtained for naturally occurring human IL-23 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IL-23.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 180 496 1170 203 526 1298
Standard Deviation 12 23.2 49.6 13.3 45.1 78.5
CV% 6.7 4.7 4.2 6.6 8.6 6

Recovery

The recovery of human IL-23 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 93 87-107
EDTA Plasma (n=4) 95 86-106
Heparin Plasma (n=4) 93 85-99
Serum (n=4) 98 88-105

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of IL-23 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human IL-23 ELISA Linearity

Scientific Data

N/A Human IL-23 ELISA Standard Curve View Larger

Human IL-23 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-23

IL-23 (Interleukin-23) is a disulfide-linked cytokine composed of a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. It is produced by activated macrophages, microglia, and monocyte-derived dendritic cells in response to pathogens. IL-23 induces the earliest recruitment of neutrophils to the site of infection and promotes the development and maintenance of Th17 cells. It also enhances the development of Th17 mediated autoimmunity and tumor progression. IL-23 signals through a receptor complex consisting of IL-12 R beta 1 and IL-23 R. This complex is expressed in mouse Th1 and Th2 cells, bone marrow dendritic cells, activated macrophages and CD4+ CD45Rb(low) memory T cells.

Long Name:
Interleukin 23
Entrez Gene IDs:
51561 (Human); 83430 (Mouse); 155140 (Rat); 102128555 (Cynomolgus Monkey)
Alternate Names:
IL-23 p19/IL-12 p40; IL23; IL-23; IL-23A; IL-23-A; IL-23p19; IL-23p19/IL-12p40; IL23P19P19; interleukin 23 p19 subunit; interleukin 23, alpha subunit p19; interleukin-23 subunit alpha; Interleukin-23 subunit p19; JKA3 induced upon T-cell activation; MGC79388; SGRF; SGRFIL-23 subunit alpha
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human IL-23 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Differential Response of Human Dendritic Cells upon Stimulation with Encapsulated or Non-Encapsulated Isogenic Strains of Porphyromonas gingivalis
    Authors: Melgar-Rodríguez, S;Polanco, A;Ríos-Muñoz, J;García, M;Sierra-Cristancho, A;González-Osuna, L;Díaz-Zúñiga, J;Carvajal, P;Vernal, R;Bravo, D;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
  2. SIRPalpha maintains macrophage homeostasis by interacting with PTK2B kinase in Mycobacterium tuberculosis infection and through autophagy and necroptosis
    Authors: D Wang, Y Lin, F Xu, H Zhang, X Zhu, Z Liu, Y Hu, G Dong, B Sun, Y Yu, G Ma, Z Tang, D Legarda, A Ting, Y Liu, J Hou, L Dong, H Xiong
    EBioMedicine, 2022-10-03;85(0):104278.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. IL-23 signaling prevents ferroptosis-driven renal immunopathology during candidiasis
    Authors: N Millet, NV Solis, D Aguilar, MS Lionakis, RT Wheeler, N Jendzjowsk, M Swidergall
    Nature Communications, 2022-09-22;13(1):5545.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Potential Protective Role of Galectin-3 in Patients with Gonarthrosis and Diabetes Mellitus: A Cross-Sectional Study
    Authors: J Milosevic, M Jurisevic, V Grbovic, I Jovanovic, N Gajovic, A Jurisic-Sk
    International Journal of Environmental Research and Public Health, 2022-09-13;19(18):.
    Species: Human
    Sample Types: Serum
  5. The Mechanism of Inflammatory Factors and Soluble Vascular Cell Adhesion Molecule-1 Regulated by Nuclear Transcription Factor NF-kappaB in Unstable Angina Pectoris
    Authors: Q Su, L Zhang, Z Qi, B Huang
    Journal of Immunology Research, 2022-07-30;2022(0):6137219.
    Species: Human
    Sample Types: Serum
  6. Nanoplastics affect the inflammatory cytokine release by primary human monocytes and dendritic cells
    Authors: A Weber, A Schwiebs, H Solhaug, J Stenvik, AM Nilsen, M Wagner, B Relja, HH Radeke
    Environment international, 2022-03-15;163(0):107173.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Expression of Circulating MicroRNAs and Myokines and Interactions with Serum Osteopontin in Type 2 Diabetic Patients with Moderate and Poor Glycemic Control: A Biochemical and Molecular Study
    Authors: HA Al-Rawaf, AH Alghadir, SA Gabr
    BioMed Research International, 2021-12-07;2021(0):7453000.
    Species: Human
    Sample Types: Serum
  8. Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia
    Authors: VR Ribeiro, M Romao-Veig, PR Nunes, ML Matias, JC Peracoli, MTS Peracoli
    International immunopharmacology, 2021-11-20;0(0):108366.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Serum Interleukin (IL)-23 and IL-17 Profile in Inflammatory Bowel Disease (IBD) Patients Could Differentiate between Severe and Non-Severe Disease
    Authors: LA Lucaciu, M Ilie?, ?C Vesa, R Seicean, S Din, CA Iuga, A Seicean
    Journal of personalized medicine, 2021-11-02;11(11):.
    Species: Human
    Sample Types: Serum
  10. Lack of Association between Serum Interleukin-23 and Interleukin-27 Levels and Disease Activity in Patients with Active Systemic Lupus Erythematosus
    Authors: K Pawlak-Bu?, W Schmidt, P Leszczy?sk
    Journal of Clinical Medicine, 2021-10-19;10(20):.
    Species: Human
    Sample Types: Serum
  11. Evaluating the Role of the Interleukin-23/17 Axis in Critically Ill COVID-19 Patients
    Authors: E Jahaj, AG Vassiliou, C Keskinidou, P Gallos, CS Vrettou, S Tsipilis, Z Mastora, SE Orfanos, I Dimopoulou, A Kotanidou
    Journal of personalized medicine, 2021-09-07;11(9):.
    Species: Human
    Sample Types: Serum
  12. Porphyromonas gingivalis lipopolysaccharide promotes T-hel per17 cell differentiation by upregulating Delta-like ligand 4 expression on CD14+ monocytes
    Authors: C Zhang, C Xu, L Gao, X Li, C Zhao
    PeerJ, 2021-04-23;9(0):e11094.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Inflammatory versus Anti-inflammatory Profiles in Major Depressive Disorders-The Role of IL-17, IL-21, IL-23, IL-35 and Foxp3
    Authors: M Ga?ecka, K Bli?niewsk, A Orzechowsk, J Szemraj, M Maes, M Berk, KP Su, P Ga?ecki
    Journal of personalized medicine, 2021-01-23;11(2):.
    Species: Human
    Sample Types: Serum
  14. 'Psoriasis 1' reduces T‑lymphocyte‑mediated inflammation in patients with psoriasis by inhibiting vitamin D receptor‑mediated STAT4 inactivation.
    Authors: Gao Y, Sun W, Cha X, Wang H
    Int J Mol Med, 2020-08-06;46(4):1538-1550.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Immune Profiling To Predict Outcome of Clostridioides difficile Infection
    Authors: MM Abhyankar, JZ Ma, KW Scully, AJ Nafziger, AL Frisbee, MM Saleh, GR Madden, AR Hays, M Poulter, WA Petri
    MBio, 2020-05-26;11(3):.
    Species: Human
    Sample Types:
  16. Mesangial Cells Exhibit Features of Antigen-Presenting Cells and Activate CD4+ T Cell Responses
    Authors: H Yu, S Cui, Y Mei, Q Li, L Wu, S Duan, G Cai, H Zhu, B Fu, L Zhang, Z Feng, X Chen
    J Immunol Res, 2019-06-17;2019(0):2121849.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Effects of early enteral nutrition on Th17/Treg cells and IL-23/IL-17 in septic patients
    Authors: JK Sun, WH Zhang, WX Chen, X Wang, XW Mu
    World J. Gastroenterol., 2019-06-14;25(22):2799-2808.
    Species: Human
    Sample Types: Serum
  18. Plasma levels of TNF-?, IL-6, IFN-?, IL-12, IL-17, IL-22, and IL-23 in achalasia, eosinophilic esophagitis (EoE), and gastroesophageal reflux disease (GERD)
    Authors: S Clayton, E Cauble, A Kumar, N Patil, D Ledford, N Kolliputi, MF Lopes-Vire, D Castell, J Richter
    BMC Gastroenterol, 2019-02-11;19(1):28.
    Species: Human
    Sample Types: Plasma
  19. PYY plays a key role in the resolution of diabetes following bariatric surgery in humans
    Authors: C Guida, SD Stephen, M Watson, N Dempster, P Larraufie, T Marjot, T Cargill, L Rickers, M Pavlides, J Tomlinson, JFL Cobbold, CM Zhao, D Chen, F Gribble, F Reimann, R Gillies, B Sgromo, P Rorsman, JD Ryan, RD Ramracheya
    EBioMedicine, 2019-01-11;0(0):.
    Species: Human
    Sample Types: Serum
  20. Three Constituents of Moringa oleifera Seeds Regulate Expression of Th17-Relevant Cytokines and Ameliorate TPA-Induced Psoriasis-Like Skin Lesions in Mice
    Authors: N Ma, Q Tang, WT Wu, XA Huang, Q Xu, GL Rong, S Chen, JP Song
    Molecules, 2018-12-10;23(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  21. Elevations of novel cytokines in bacterial meningitis in infants
    Authors: L Srinivasan, L Kilpatrick, SS Shah, S Abbasi, MC Harris
    PLoS ONE, 2018-02-02;13(2):e0181449.
    Species: Human
    Sample Types: CSF
  22. Increased Interleukin-23 in Hashimoto's Thyroiditis Disease Induces Autophagy Suppression and Reactive Oxygen Species Accumulation
    Authors: T Zheng, C Xu, C Mao, X Mou, F Wu, X Wang, L Bu, Y Zhou, X Luo, Q Lu, H Liu, G Yuan, S Wang, D Chen, Y Xiao
    Front Immunol, 2018-01-29;9(0):96.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. MMP-8 and TIMP-1 are associated to periodontal inflammation in patients with rheumatoid arthritis under methotrexate immunosuppression - First results of a cross-sectional study
    Authors: G Schmalz, I Davarpanah, J Jäger, RF Mausberg, B Krohn-Grim, J Schmidt, R Haak, U Sack, D Ziebolz
    J Microbiol Immunol Infect, 2017-09-04;0(0):.
    Species: Human
    Sample Types: Serum
  24. High serum resistin associates with intrahepatic inflammation and necrosis: an index of disease severity for patients with chronic HBV infection
    Authors: Z Meng, Y Zhang, Z Wei, P Liu, J Kang, Y Zhang, D Ma, C Ke, Y Chen, J Luo, Z Gong
    BMC Gastroenterol, 2017-01-07;17(1):6.
    Species: Human
    Sample Types: Serum
  25. Age-Specific Adjuvant Synergy: Dual TLR7/8 and Mincle Activation of Human Newborn Dendritic Cells Enables Th1 Polarization
    Authors: Ofer Levy
    J. Immunol., 2016-10-28;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Effects of anticipated neonatal surgical intervention on maternal milk cytokine production
    Authors: Jennifer L Liedel
    J. Pediatr. Surg., 2016-10-27;0(0):.
    Species: Human
    Sample Types: Milk
  27. Serum Interleukin-18, Fetuin-A, Soluble Intercellular Adhesion Molecule-1, and Endothelin-1 in Ankylosing Spondylitis, Psoriatic Arthritis, and SAPHO Syndrome
    Int J Mol Sci, 2016-08-03;17(8):.
    Species: Human
    Sample Types: Serum
  28. Co-culture of human bronchial fibroblasts and CD4+ T cells increases Th17 cytokine signature.
    Authors: Loubaki L, Hadj-Salem I, Fakhfakh R, Jacques E, Plante S, Boisvert M, Aoudjit F, Chakir J
    PLoS ONE, 2013-12-05;8(12):e81983.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Adenoma-linked barrier defects and microbial products drive IL-23/IL-17-mediated tumour growth.
    Authors: Grivennikov S, Wang K, Mucida D, Stewart C, Schnabl B, Jauch D, Taniguchi K, Yu G, Osterreicher C, Hung K, Datz C, Feng Y, Fearon E, Oukka M, Tessarollo L, Coppola V, Yarovinsky F, Cheroutre H, Eckmann L, Trinchieri G, Karin M
    Nature, 2012-11-08;491(7423):254-8.
    Species: Human
    Sample Types: Tissue Homogenates
  30. Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
    Authors: Nakajima H, Nakajima K, Tarutani M, Morishige R, Sano S
    Arch. Dermatol. Res., 2011-06-17;303(6):451-5.
    Species: Human
    Sample Types: Serum
  31. HDAC inhibition by LBH589 affects the phenotype and function of human myeloid dendritic cells.
    Authors: Song W, Tai YT, Tian Z, Hideshima T, Chauhan D, Nanjappa P, Exley MA, Anderson KC, Munshi NC
    Leukemia, 2010-11-19;25(1):161-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. CD14-independent responses induced by a synthetic lipid A mimetic.
    Authors: Legat A, Thomas S, Hermand P, Van Mechelen M, Goldman M, De Wit D
    Eur J Immunol, 2010-03-01;40(3):797-802.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009-03-02;119(10):1424-32.
    Species: Human
    Sample Types: Plasma
  34. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis.
    Authors: Chi W, Zhu X, Yang P, Liu X, Lin X, Zhou H, Huang X, Kijlstra A
    Invest. Ophthalmol. Vis. Sci., 2008-07-01;49(7):3058-64.
    Species: Human
    Sample Types: Serum

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Human IL-23 Quantikine ELISA Kit
By Anonymous on 10/26/2015
Sample Tested: Dendritic cells Species: Human

Standard curve is shown.
Buffer: kit dilution buffer
Dilution: 1/100

Human IL-23 Quantikine ELISA Kit D2300B