Human IL-2 R beta PE-conjugated Antibody

Catalog #: FAB224P
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB224P

Detection of IL‑2 R beta in Human Blood Lymphocytes by Flow Cytometry.

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All IL-2 R beta Antibodies

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Citations (6)

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Human IL-2 R beta PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-2 R beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL‑2 R alpha, rh gamma _c, rhIL‑4 R, or rhIL‑6 R is observed.

Source

Monoclonal Mouse IgG₁ Clone # 27302

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑2 R beta
Ala27-Asp239
Accession # NP_000869

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of IL‑2 R beta in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL-2 R beta PE-conjugated Monoclonal Antibody (Catalog # FAB224P) and Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-2 R beta

Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding, while the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid (aa) precursor Type I membrane protein with a 26 aa signal peptide, a 214 aa extracellular region, a 25 aa transmembrane region and a 286 aa cytoplasmic domain. A soluble IL-2 R beta has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. Soluble IL-2 R beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, soluble IL-2 R beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities.

Long Name

Interleukin 2 Receptor beta

Entrez Gene IDs

3560 (Human); 16185 (Mouse)

Alternate Names

CD122 antigen; CD122; high affinity IL-2 receptor beta subunit; High affinity IL-2 receptor subunit beta; IL-15 R beta; IL-2 R beta; IL-2 receptor subunit beta; IL2R beta; IL-2R subunit beta; IL2RB; IL-2Rb; interleukin 15 receptor, beta; interleukin 2 receptor, beta; interleukin-2 receptor subunit beta; P70-75; p75

Product Datasheets

Product Datasheet

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Citations for Human IL-2 R beta PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Inhibition of TGF-beta 1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-beta 1-Induced EMT in Renal Fibrosis
Authors: Aurore Devocelle, Lola Lecru, Hélène François, Christophe Desterke, Cindy Gallerne, Pierre Eid et al.
International Journal of Cell Biology
Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
J Immunol, 2013-12-02;192(1):123-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Identification of human NK17/NK1 cells.
Authors: Pandya AD, Al-Jaderi Z, Hoglund RA, Holmoy T, Harbo HF, Norgauer J, Maghazachi AA
PLoS ONE, 2011-10-21;6(10):e26780.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
Biochem. Pharmacol., 2009-08-07;79(2):188-97.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients.
Authors: Zheng CF, Jones GJ, Shi M, Wiseman JC, Marr KJ, Berenger BM, Huston SM, Gill MJ, Krensky AM, Kubes P, Mody CH
J. Immunol., 2008-06-01;180(11):7221-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Histone deacetylase inhibitors suppress natural killer cell cytolytic activity.
Authors: Ogbomo H, Michaelis M, Kreuter J, Doerr HW, Cinatl J
FEBS Lett., 2007-03-01;581(7):1317-22.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry