Human IL-2 PE-conjugated Antibody

Catalog #: IC202P
5 Citations Datasheet / COA / SDS

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IC202P

Detection of IL‑2 in Human PBMCs by Flow Cytometry.

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All IL-2 Antibodies

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Citations (5)

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Human IL-2 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-2 in direct ELISAs. Does not cross-react with recombinant IL-2 from mouse, rat, pig, or cotton rat.

Source

Monoclonal Mouse IgG₁ Clone # 5334

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human IL‑2
Ala21-Thr153
Accession # NP_000577

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of IL-2 antibody in Human PBMCs antibody by Flow Cytometry.

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Detection of IL‑2 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and ionomycin were stained with Mouse Anti-Human IL-2 PE-conjugated Monoclonal Antibody (Catalog # IC202P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-2

IL-2 is a 15-19 kDa secreted O-glycosylated polypeptide that belongs to the gamma _c -receptor utilizing family of molecules. It is a monomer that is expressed by a limited number of cell types, including CD4⁺ and CD8⁺ T cells, gamma δ T cells, eosinophils and B cells. Its local concentration, plus the stoichiometry of it receptor ( a two or three subunit complex) appears to determine what effects it has on its target cells. It is suggested to both induce NK and CD8⁺ T cell proliferation, and promote NK cell and CD8⁺ T cell effector activity. IL-2 also appears to drive CD4⁺ Fox P3^- thymocytes into mature FoxP3⁺ Tregs, and to direct the conversion of CD4⁺ T cells into induced Tregs. Finally, IL-2 induces gamma δ T cells to secrete IFN-gamma, and endothelial cells to upregulate endocytic activity. Human and mouse IL-2 share 56 % amino acid sequence identity.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IL-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Building and optimizing a virus-specific T cell receptor library for targeted immunotherapy in viral infections.
Authors: Banu, Nasirah, Chia, Adeline, Ho, Zi Zong, Garcia, Alfonso, Paravasivam, Komathi, Grotenbreg, Gijsbert, Bertoletti, Antonio, Gehring, Adam J
Sci Rep, 2014-02-25;4(0):4166.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Qualitatively different T cell phenotypic responses to IL-2 versus IL-15 are unified by identical dependences on receptor signal strength and duration.
Authors: Arneja A, Johnson H, Gabrovsek L, Lauffenburger D, White F
J Immunol, 2013-12-02;192(1):123-35.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Hypoxia potentiates glioma-mediated immunosuppression.
Authors: Wei J, Wu A, Kong LY, Wang Y, Fuller G, Fokt I, Melillo G, Priebe W, Heimberger AB
PLoS ONE, 2011-01-20;6(1):e16195.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human CD4(+) T cells recognize an epitope within alpha-fetoprotein sequence and develop into TGF-beta-producing CD4(+) T cells.
Authors: Alisa A, Boswell S, Pathan AA, Ayaru L, Williams R, Behboudi S
J. Immunol., 2008-04-01;180(7):5109-17.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
Authors: Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
Cancer Res., 2007-10-15;67(20):9630-6.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry