Human IL-2 Magnetic Luminex® Performance Assay

Name: Human IL-2 Magnetic Luminex® Performance Assay
Brand: R&D Systems
SKU: LUHM202
Price: 189 USD

Catalog #: LUHM202
2 Citations Datasheet / COA / SDS

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Quantist software graph of signal intensity versus concentration for IL-2 in the Human Cytokine A Luminex Panel

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Summary Configure Your Assay Product Features Precision & Accuracy Data Examples Product Datasheets Background

Human IL-2 Magnetic Luminex® Performance Assay Summary

Panel	Luminex Performance Human Cytokine Panel A
Assay Type	Magnetic bead-based multiplex assay for the Luminex^® platform
Format	1 x 96-well microplate and Magnetic antibody-coated beads
Species	Human
Analytes Detected	Please see analyte list in assay customization tool below.
Performance Validation	Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human IL-2 with up to analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine^® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Base Kit Components

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents

User-mix antibody-coated Magnetic beads in individual vials
User-mixed cocktail of biotinylated detection antibody

Precision

	Intra-Assay Precision			Inter-Assay Precision
Intra-assay Precision (precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (precision between assays) - Three samples of known concentration were tested in twenty separate assays to assess precision between assays.
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	10	23	203	28	265	543
Standard deviation	0.52	1.19	6.39	3.7	34	52.5
CV (%)	5.2	5.2	3.1	13.2	12.8	9.7

Recovery

Sample Type	Average % Recovery	Range (%)
Samples containing and/or spiked with human IL-2 were evaluated for recovery.
Cell culture supernate	94	89-98
Serum	109	98-119
EDTA plasma	100	88-111
Heparin plasma	103	90-123

Linearity

		Cell culture supernate	Serum	EDTA plasma	Heparin plasma
Samples containing and/or spiked with human IL-2 were serially diluted to evaluate assay linearity.
1:2	Average % of Expected	101	103	104	100
1:2	Range (%)	89-116	99-106	97-109	88-118
1:4	Average % of Expected	102	99	100	93
1:4	Range (%)	86-124	88-109	91-104	80-114
1:8	Average % of Expected	102	95	96	90
1:8	Range (%)	84-121	80-112	89-102	81-105

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex^® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

N/A

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Quantist software graph of signal intensity versus concentration for IL-2 in the Human Cytokine A Luminex Panel

Background: IL-2

IL-2 (Interleukin 2) is a critical cytokine in T cell biology. It promotes T cell activation and expansion, the development, maintenance and function of regulatory T cells (Treg), and the differentiation of CD8+ T cells into terminal effector cells and memory cells. IL-2 signals through a receptor complex composed of CD25/IL-2 R alpha, IL-2 R beta, and the Common gamma Chain (gamma c). IL-2 R beta is also a component of the IL-15 receptor complex. Gamma c is also a signaling subunit in the receptors for IL-4, -7, -9, -15, and -21.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Product Datasheets

COA

SDS

Citations for Human IL-2 Magnetic Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies
Authors: Chen, S;Nguyen, TD;Lee, KZ;Liu, D;
Biotechnology advances
Species: Human
Sample Types: Cell Culture Supernates
Inactivated Vaccine-Induced SARS-CoV-2 Variant-Specific Immunity in Children
Authors: JA Soto, F Melo-Gonzá, C Gutierrez-, BM Schultz, RV Berríos-Ro, D Rivera-Pér, A Piña-Iturb, G Hoppe-Elsh, LF Duarte, Y Vázquez, D Moreno-Tap, M Ríos, PA Palacios, R Garcia-Bet, Á Santibañez, GA Pacheco, C Mendez, CA Andrade, PH Silva, B Diethelm-V, P Astudillo, M Calvo, A Cárdenas, M González, M Goldsack, V Gutiérrez, M Potin, A Schilling, LI Tapia, L Twele, R Villena, A Grifoni, A Sette, D Weiskopf, RA Fasce, J Fernández, J Mora, E Ramírez, A Gaete-Arge, ML Acevedo, F Valiente-E, R Soto-Rifo, A Retamal-Dí, N Muñoz-Jofr, PedCoronaV, X Meng, Q Xin, E Alarcón-Bu, JV González-A, N Le Corre, MJ Álvarez-Fi, PA González, K Abarca, C Perret, LJ Carreño, SM Bueno, AM Kalergis
MBio, 2022-11-16;0(0):e0131122.
Species: Human
Sample Types: Cell Culture Supernates