Human IL-15 Biotinylated Antibody

Catalog #: BAF247
4 Citations Datasheet / COA / SDS

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BAF247

All IL-15 Antibodies

Product Details

Citations (4)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-15 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-15 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse IL-15 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IL‑15 (Catalog # 247-IL)

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells.
IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Eight isoforms of
IL‑15 R alpha mRNA have been identified resulting from alternative splicing events involving different exons. The exclusion of exon 2 results in an IL-15 R alpha isoform that does not bind IL-15. Human IL-15 R alpha DE3 cDNA encodes a 267 amino acid (aa) protein that contains a 30 aa signal sequence, a 175 aa extracellular region containing one N-linked glycosylation site, a 21 aa transmembrane domain, and a 41 aa cytoplasmic tail. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma _c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References

Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.

Long Name

Interleukin 15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Alternate Names

IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IL-15 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Authors: Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood, 2007-02-08;109(10):4272-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development
Marked differences in human melanoma antigen-specific T cell responsiveness after vaccination using a functional microarray.
Authors: Chen DS, Soen Y, Stuge TB
PLoS Med., 2005-09-20;2(10):e265.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay
IL-21 sustains CD28 expression on IL-15-activated human naive CD8+ T cells.
Authors: Alves NL, Arosa FA, van Lier RA
J. Immunol., 2005-07-15;175(2):755-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Renal cells express a functional interleukin-15 receptor.
Authors: Tejman-Yarden N, Zlotnik M, Lewis E, Etzion O, Chaimovitz C, Douvdevani A
Nephrol. Dial. Transplant., 2005-01-25;20(3):516-23.
Species: Human
Sample Types: Tissue Homogenates
Applications: Dot Blot