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Human IL-15 Antibody

Catalog #: AF315
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF315
AF315-SP
IL‑15 in Human Tonsil.
2 Images
Product Details
Citations (11)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-15 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-15 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse IL‑15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑15 (Catalog # 247-IL)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line [Avanzi, G. et al. (1988) Br. J. Haematol. 69:359]. The Neutralization Dose (ND50) is typically 0.5-1.5 µg/mL in the presence of 10 ng/mL of Recombinant Human IL‑15.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry IL‑15 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

IL‑15 in Human Tonsil. IL‑15 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. View Larger

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL-15 (Catalog # 247-IL) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-15 (10 ) is neutralized (green line) by increasing concen-trations of Goat Anti-Human IL-15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF315). The ND50 is typically 0.5-1.5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References
  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
Long Name
Interleukin 15
Entrez Gene IDs
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

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Citations for Human IL-15 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. A First-in-Human Phase 1 Study of Subcutaneous Outpatient Recombinant Human IL-15 (rhIL-15) in Adults with Advanced Solid Tumors
    Authors: JS Miller, C Morishima, DG McNeel, MR Patel, HE Kohrt, JA Thompson, PM Sondel, HA Wakelee, ML Disis, JC Kaiser, MA Cheever, H Streicher, SP Creekmore, TA Waldmann, KC Conlon
    Clin. Cancer Res., 2017-12-04;0(0):.
  2. Discovery and characterization of a novel humanized anti-IL-15 antibody and its relevance for the treatment of refractory celiac disease and eosinophilic esophagitis
    Authors: AP Vicari, AM Schoepfer, B Meresse, L Goffin, O Léger, S Josserand, N Guégan, S Yousefi, A Straumann, N Cerf-Bensu, HU Simon, Y Chvatchko
    MAbs, 2017-06-05;0(0):0.
  3. Efficient production and enhanced tumor delivery of engineered extracellular vesicles
    Authors: Dionysios C. Watson, Defne Bayik, Avinash Srivatsan, Cristina Bergamaschi, Antonio Valentin, Gang Niu et al.
    Biomaterials
  4. Recombinant human heterodimeric IL-15 complex displays extensive and reproducible N- and O-linked glycosylation
    Authors: M. Thaysen-Andersen, E. Chertova, C. Bergamaschi, E. S. X. Moh, O. Chertov, J. Roser et al.
    Glycoconjugate Journal
  5. TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells.
    Authors: Mongini P, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz J, Allen S, Rai K, Chu C, Chiorazzi N
    J Immunol, 2015-07-01;195(3):901-23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis.
    Authors: Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N
    J Immunol, 2015-01-23;194(5):2099-109.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  7. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Enrichment and persistence of virus-specific CTL in the brain of simian immunodeficiency virus-infected monkeys is associated with a unique cytokine environment.
    Authors: Marcondes MC, Burdo TH, Sopper S, Huitron-Resendiz S, Lanigan C, Watry D, Flynn C, Zandonatti M, Fox HS
    J. Immunol., 2007-05-01;178(9):5812-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Dendritic cells induce MUC1 expression and polarization on human T cells by an IL-7-dependent mechanism.
    Authors: Vasir B, Avigan D, Wu Z, Crawford K, Turnquist S, Ren J, Kufe D
    J. Immunol., 2005-02-15;174(4):2376-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Cloning and expression of feline interleukin 15.
    Authors: Dean GA, Barger A, Lavoy A
    Cytokine, 2005-01-21;29(2):77-83.
    Species: Feline
    Sample Types: Whole Cells
    Applications: ICC, Western Blot
  11. NK cells regulate CD8+ T cell effector function in response to an intracellular pathogen.
    Authors: Vankayalapati R, Klucar P, Wizel B, Weis SE, Samten B, Safi H, Shams H, Barnes PF
    J. Immunol., 2004-01-01;172(1):130-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Human IL-15 Antibody
By Anonymous on 05/01/2024
Application: Flow Sample Tested: U-87 MG human glioblastoma/astrocytoma cell line Species: Human
Flow Cytometry Human IL-15 Antibody AF315