Human IL-1 RII Antibody Summary
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2-dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15‑30 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.
Product Datasheets
Citations for Human IL-1 RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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IL-1R2 expression in human gastric cancer and its clinical significance
Authors: M Yuan, L Wang, H Huang, Y Li, X Zheng, Q Shao, J Jiang
Bioscience Reports, 2021-03-26;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC -
Human gastrointestinal epithelia of the esophagus, stomach, and duodenum resolved at single-cell resolution
Authors: GA Busslinger, BLA Weusten, A Bogte, H Begthel, LAA Brosens, H Clevers
Cell Reports, 2021-03-09;34(10):108819.
Species: Human
Sample Types: Organoid
Applications: IHC -
Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
Authors: Chang SY, Su PF, Lee TC
Cytokine, 2008-11-20;45(1):32-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Authors: Herrmann-Lavoie C, Rao CV, Akoum A
Endocrinology, 2007-08-16;148(11):5377-84.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
Authors: Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
Invest. Ophthalmol. Vis. Sci., 2007-06-01;48(6):2634-43.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Estrogen’s bone-protective effects may involve differential IL-1 receptor regulation in human osteoclast-like cells
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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MAB663 was used as the capture and AF263 was used as the detection antibody for the sandwich ELISA for sIL-1R2. The immunoassay standard was 263-2R. Assay had sensitivity of ~10pg/ml.
