Human IL-1 RII Antibody

Catalog #: MAB2631 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2631
MAB2631-SP

Detection of Human IL‑1 RII by Western Blot.

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All IL-1 RII Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-1 RII Antibody Summary

Species Reactivity

Human

Specificity

Detects rhIL-1 RII in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-18 R or recombinant mouse IL-18 R is observed.

Source

Monoclonal Mouse IgG₁ Clone # 34117

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL‑1 RII by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB2631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 RII at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References

Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.

Long Name

Interleukin 1 Receptor II

Entrez Gene IDs

7850 (Human); 16178 (Mouse)

Alternate Names

beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725