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Human IgG Fc APC-conjugated Antibody

Catalog #: FAB110A
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB110A
FAB110A-100
Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.
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Citations (6)
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Human IgG Fc APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 97924
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of IgG<SUB>1</SUB>Fc antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of IgG1Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry. Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with either (A) Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). Panel C shows HEK293 cell line transfected with human PD-1, untreated with B7-H1 Fc Chimera, and stained with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Human IgG Fc APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. ZL-1211 Exhibits Robust Antitumor Activity by Enhancing ADCC and Activating NK Cell–mediated Inflammation in CLDN18.2-High and -Low Expressing Gastric Cancer Models
    Authors: Hiroyasu Konno, Tracey Lin, Renyi Wu, Xinchuan Dai, Shou Li, Grace Wang et al.
    Cancer Research Communications
  2. Early Reconstitution of Antibody Secreting Cells after Allogeneic Stem Cell Transplantation
    Authors: M Hinterleit, C Hinterleit, E Malenke, B Federmann, U Holzer, M Müller, WA Bethge, S Wirths
    Journal of Clinical Medicine, 2022-01-05;11(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Evolutionary pathways to SARS-CoV-2 resistance are opened and closed by�epistasis acting on ACE2
    Authors: GM Castiglion, L Zhou, Z Xu, Z Neiman, CF Hung, EJ Duh
    PloS Biology, 2021-12-21;19(12):e3001510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Approaches to overcome flow cytometry limitations in the analysis of cells from veterinary relevant species
    Authors: J Hunka, JT Riley, GF Debes
    BMC Vet. Res., 2020-03-06;16(1):83.
    Species: Sheep
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Arming oHSV with ULBP3 drives abscopal immunity in lymphocyte-depleted glioblastoma
    Authors: HG Wirsching, H Zhang, F Szulzewsky, S Arora, P Grandi, PJ Cimino, N Amankulor, JS Campbell, L McFerrin, SS Pattwell, C Ene, A Hicks, M Ball, J Yan, J Zhang, D Kumasaka, RH Pierce, M Weller, M Finer, C Quéva, JC Glorioso, AM Houghton, EC Holland
    JCI Insight, 2019-07-11;4(13):.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Frequency and role of NKp46 and NKG2A in hepatitis B virus infection
    Authors: T Yoshioka, T Tatsumi, T Miyagi, K Mukai, K Nishio, A Nishio, Y Yokoyama, T Suda, T Kegasawa, M Shigekawa, H Hikita, R Sakamori, T Takehara
    PLoS ONE, 2017-03-22;12(3):e0174103.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. What is the light chain of the Human IgG Fc Antibody, Catalog #s MAB110 and FAB110, Clone # 97924?

    • Catalog #s MAB110 and FAB110 have a kappa light chain.

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