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Human IGF-I/IGF-1 Antibody

Catalog #: MAB291
7 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB291-SP
MAB291-100
MAB291-500
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Detection of Recombinant Human IGF-I/IGF-1 by Western Blot.
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Citations (7)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IGF-I/IGF-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.
Source
Monoclonal Mouse IgG1 Clone # 56408
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Human IGF-I/IGF-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF291)

Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human IGF-I/IGF-1 antibody by Western Blot. View Larger

Detection of Recombinant Human IGF-I/IGF-1 by Western Blot. Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C

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Citations for Human IGF-I/IGF-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. ATM-dependent IGF-1 induction regulates secretory clusterin expression after DNA damage and in genetic instability
    Authors: E M Goetz, B Shankar, Y Zou, J C Morales, X Luo, S Araki et al.
    Oncogene
  2. Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei.
    Authors: Landowski C, Huuskonen A, Wahl R, Westerholm-Parvinen A, Kanerva A, Hanninen A, Salovuori N, Penttila M, Natunen J, Ostermeier C, Helk B, Saarinen J, Saloheimo M
    PLoS ONE, 2015-08-26;10(8):e0134723.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot
  3. ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence.
    Authors: Luo X, Suzuki M, Ghandhi S, Amundson S, Boothman D
    PLoS ONE, 2014-06-17;9(6):e99983.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Capture)
  4. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development
  5. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007-02-01;13(3):1000-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  6. Construction of Plasmid DNA Expressing Two Isoforms of Insulin-Like Growth Factor-1 and Its Effects on Skeletal Muscle Injury Models
    Authors: Jaeman Lee, Kyeong-Ryang Ko, Nayeon Lee, Sojin Kim, Seung-Shin Yu, Sunyoung Kim et al.
    Human Gene Therapy

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Reviews for Human IGF-I/IGF-1 Antibody

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Human IGF-I/IGF-1 Antibody
By Anonymous on 06/18/2021
Application: ELISA Sample Tested: cells medium,HE49 cells Species: Human

Samples were normalized to cell number and the total volume of medium

ELISA Human IGF-I/IGF-1 Antibody MAB291
Human IGF-I/IGF-1 Antibody
By Anonymous on 04/10/2021
Application: IHC Sample Tested: decidual Species: Human

Low primary antibody dilution (1:500) for IHC.

Immunohistochemistry Human IGF-I/IGF-1 Antibody MAB291