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Human IFN-gamma  R1/CD119 Antibody

Catalog #: AF673
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF673
AF673-SP
IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IFN-gamma  R1/CD119 Antibody Summary

Species Reactivity
Human
Specificity
Detects IFN‑ gamma  R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
IFN-gamma  R2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IFN-gamma  R1/CD119
Glu18-Gly245
Accession # P15260
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IFN-gamma  R1/CD119 (Catalog # 673-IR)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IFN‑ gamma  R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.5-2 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. View Larger

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673). The ND50 is typically 0.5-2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma  receptor complex is made up of two type I membrane proteins, IFN-gamma  R1 (IFN-gamma  R alpha ) and IFN-gamma  R2 (IFN-gamma  R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma  R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma  R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma  R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma  R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma  R1 is constitutively expressed in most cell types. Soluble IFN-gamma  R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma  R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

References
  1. Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.
Long Name
Interferon gamma Receptor 1
Entrez Gene IDs
3459 (Human); 15979 (Mouse)
Alternate Names
AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

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Citations for Human IFN-gamma  R1/CD119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy
    Authors: RV Broad, SJ Jones, MC Teske, LM Wastall, AM Hanby, JL Thorne, TA Hughes
    British Journal of Cancer, 2021-01-04;0(0):.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  2. Interferon-gamma and Smac mimetics synergize to induce apoptosis of lung cancer cells in a TNF alpha -independent manner
    Authors: Qin Hao, Hua Tang
    Cancer Cell International
  3. Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection
    Authors: SY Zhang, NE Clark, CA Freije, E Pauwels, AJ Taggart, S Okada, H Mandel, P Garcia, MJ Ciancanell, A Biran, FG Lafaille, M Tsumura, A Cobat, J Luo, S Volpi, B Zimmer, S Sakata, A Dinis, O Ohara, EJ Garcia Rei, K Dobbs, M Hasek, SP Holloway, K McCammon, SA Hussong, N DeRosa, CE Van Skike, A Katolik, L Lorenzo, M Hyodo, E Faria, R Halwani, R Fukuhara, GA Smith, V Galvan, MJ Damha, S Al-Muhsen, Y Itan, JD Boeke, LD Notarangel, L Studer, M Kobayashi, L Diogo, WG Fairbrothe, L Abel, BR Rosenberg, PJ Hart, A Etzioni, JL Casanova
    Cell, 2018-02-22;172(5):952-965.e18.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  4. Plasma from systemic lupus patients compromises cholesterol homeostasis: a potential mechanism linking autoimmunity to atherosclerotic cardiovascular disease
    Authors: Allison B. Reiss, Kamran Anwar, Joan T. Merrill, Edwin S. L. Chan, Nahel W. Awadallah, Bruce N. Cronstein et al.
    Rheumatology International
  5. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009-03-02;119(10):1424-32.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor.
    Authors: Saidi RF, Williams F, Silberberg B, Mittal VK, ReMine SG, Jacobs MJ
    Surgery, 2006-06-01;139(6):743-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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