Human IFN-gamma R1/CD119 Antibody

Catalog #: AF673
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF673
AF673-SP

IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody.

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Citations (6)

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Human IFN-gamma R1/CD119 Antibody Summary

Species Reactivity

Human

Specificity

Detects IFN‑ gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human
IFN-gamma R2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IFN-gamma R1/CD119
Glu18-Gly245
Accession # P15260

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human IFN-gamma R1/CD119 (Catalog # 673-IR)

Immunocytochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND₅₀) is typically 0.5-2 µg/mL in the presence of 2 ng/mL Recombinant Human IFN‑ gamma.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ gamma R1/CD119 Antibody. Recombinant Human IFN-gamma (Catalog # 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IFN-gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF673). The ND₅₀ is typically 0.5-2 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-gamma R1/CD119

The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist.

References

Bach, E.A. et al. (1997) Annu. Rev. Immunol. 15:563.

Long Name

Interferon gamma Receptor 1

Entrez Gene IDs

3459 (Human); 15979 (Mouse)

Alternate Names

AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma R1; IFN-gamma receptor 1; IFNgammaR1; IFN-gamma-R1; IFNGR; IFNGR1; IFN-gR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human IFN-gamma R1/CD119 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Inhibition of interferon-signalling halts cancer-associated fibroblast-dependent protection of breast cancer cells from chemotherapy
Authors: RV Broad, SJ Jones, MC Teske, LM Wastall, AM Hanby, JL Thorne, TA Hughes
British Journal of Cancer, 2021-01-04;0(0):.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
Interferon-gamma and Smac mimetics synergize to induce apoptosis of lung cancer cells in a TNF alpha -independent manner
Authors: Qin Hao, Hua Tang
Cancer Cell International
Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection
Authors: SY Zhang, NE Clark, CA Freije, E Pauwels, AJ Taggart, S Okada, H Mandel, P Garcia, MJ Ciancanell, A Biran, FG Lafaille, M Tsumura, A Cobat, J Luo, S Volpi, B Zimmer, S Sakata, A Dinis, O Ohara, EJ Garcia Rei, K Dobbs, M Hasek, SP Holloway, K McCammon, SA Hussong, N DeRosa, CE Van Skike, A Katolik, L Lorenzo, M Hyodo, E Faria, R Halwani, R Fukuhara, GA Smith, V Galvan, MJ Damha, S Al-Muhsen, Y Itan, JD Boeke, LD Notarangel, L Studer, M Kobayashi, L Diogo, WG Fairbrothe, L Abel, BR Rosenberg, PJ Hart, A Etzioni, JL Casanova
Cell, 2018-02-22;172(5):952-965.e18.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Capture
Plasma from systemic lupus patients compromises cholesterol homeostasis: a potential mechanism linking autoimmunity to atherosclerotic cardiovascular disease
Authors: Allison B. Reiss, Kamran Anwar, Joan T. Merrill, Edwin S. L. Chan, Nahel W. Awadallah, Bruce N. Cronstein et al.
Rheumatology International
Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
Circulation, 2009-03-02;119(10):1424-32.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Expression of interferon receptors in pancreatic cancer: identification of a novel prognostic factor.
Authors: Saidi RF, Williams F, Silberberg B, Mittal VK, ReMine SG, Jacobs MJ
Surgery, 2006-06-01;139(6):743-8.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P