Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

Name: Human IFN-gamma Quantikine HS ELISA Kit
Brand: R&D Systems
SKU: HSDIF0
Rating: 5 (2 reviews)

A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

Human IFN-gamma Quantikine HS ELISA Kit

Catalog #: HSDIF0
2 Citations 2 Reviews Datasheet / COA / SDS

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HSDIF0

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Human IFN-gamma HS Quantikine ELISA Standard Curve

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Product Details

Citations (2)

FAQs

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Reviews (2)

Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IFN-gamma Quantikine HS ELISA Kit Summary

Assay Length

4.0 hours

Sample Type & Volume Required Per Well

Serum (100 uL), EDTA Plasma ( Not Assigned), Heparin Plasma (100 uL)

Sensitivity

0.173 pg/mL

Assay Range

0.5 - 30 pg/mL (Serum, Plasma, EDTA Plasma, Heparin Plasma)

Specificity

Natural and recombinant human IFN-gamma.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Product Summary

The Quantikine^® HS Human IFN-gamma Immunoassay is a 4.0 hour solid-phase ELISA designed to measure human IFN-gamma in serum and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant protein. Results obtained using natural human IFN-gamma showed linear curves that were parallel to the standard curves obtained using the Quantikine^® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians

Serum, Plasma, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	1.83	6.1	12.8	2.15	6.33	12.4
Standard Deviation	0.084	0.187	0.253	0.21	0.421	0.898
CV%	4.6	3.1	2	9.8	6.7	7.2

Recovery

The recovery of human IFN-gamma spiked to levels throughout the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	99	90-108
Heparin Plasma (n=4)	99	92-109
Serum (n=4)	97	85-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human IFN-gamma were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

N/A

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Human IFN-gamma HS Quantikine ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:

Interferon gamma

Entrez Gene IDs:

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names:

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Related Research Areas

Citations for Human IFN-gamma Quantikine HS ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Interferon and interferon-induced cytokines as markers of impending clinical progression in ANA+ individuals without a systemic autoimmune rheumatic disease diagnosis
Authors: ST Kim, C Muñoz-Graj, SE Dunn, R Schneider, SR Johnson, Z Touma, Z Ahmad, D Bonilla, EG Atenafu, LT Hiraki, A Bookman, J Wither
Arthritis Research & Therapy, 2023-02-10;25(1):21.
Species: Human
Sample Types: Serum
The prognostic significance of serum interferon-gamma (IFN-gamma) in hormonally dependent breast cancer
Authors: N Todorovi?-, J Milovanovi, J Greenman, M Radulovic
Cytokine, 2022-02-23;152(0):155836.
Species: Human
Sample Types: Serum

FAQs

Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
- A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

View all ELISA FAQs