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Human IFN-alpha Antibody

Catalog #: 21100-1
13 Citations Datasheet / COA / SDS
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21100-1
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Citations (13)
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Human IFN-alpha Antibody Summary

Species Reactivity
Human
Specificity
Neutralizes human interferon alpha; binds to human interferon alpha with high affinity (KA ≈ 109 M-1)
Source
Monoclonal Mouse IgG1 Clone # MMHA-2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human Leukocyte Interferon
Formulation
Phosphate-buffered saline (PBS) containing 0.1% bovine serum albumin (BSA).
Endotoxin Level
<1.0 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilutions should be determined by each laboratory for each application.
 
ELISA
Optimal dilutions should be determined by each laboratory for each application.
 
Neutralization
One neutralization unit is the amount of antiserum required to neutralize one unit of human interferon alpha (Hu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Famille tti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758; Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods in Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon, about 1 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Hu-IFN-alpha provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23].

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use ( ≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Re-freezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.

Long Name
Interferon alpha
Entrez Gene IDs
3440 (Human)
Alternate Names
IFNA; IFNA2; IFNA2b; IFNalpha; IFN-alpha; IFN-alphaA; INFA2; interferon alpha 2; LeIF D

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Citations for Human IFN-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Autoantibodies neutralizing type I IFNs underlie West Nile virus encephalitis in ∼40% of patients
    Authors: Adrian Gervais, Francesca Rovida, Maria Antonietta Avanzini, Stefania Croce, Astrid Marchal, Shih-Ching Lin et al.
    Journal of Experimental Medicine
  2. Autoantibodies neutralizing type I IFNs are present in ~4% of uninfected individuals over 70 years old and account for ~20% of COVID-19 deaths
    Authors: Paul Bastard, Adrian Gervais, Tom Le Voyer, Jérémie Rosain, Quentin Philippot, Jérémy Manry et al.
    Sci Immunol
  3. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
    Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
    Cancer Immunology Research
  4. A novel variant in AIRE causing a rare, non‑classical autoimmune polyendocrine syndrome type 1
    Authors: Wen‑Bin Zheng, Lu‑Jiao Li, Di‑Chen Zhao, Ou Wang, Yan Jiang, Wei‑Bo Xia et al.
    Molecular Medicine Reports
  5. Auto-antibodies to type I IFNs can underlie adverse reactions to yellow fever live attenuated vaccine
    Authors: Paul Bastard, Eleftherios Michailidis, Hans-Heinrich Hoffmann, Marwa Chbihi, Tom Le Voyer, Jérémie Rosain et al.
    Journal of Experimental Medicine
  6. Antigen delivery to dendritic cells shapes human CD4+ and CD8+ T cell memory responses to Staphylococcus aureus
    Authors: J Uebele, C Stein, MT Nguyen, A Schneider, F Kleinert, O Tichá, G Bierbaum, F Götz, I Bekeredjia
    PLoS Pathog., 2017-05-25;13(5):e1006387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors.
    Authors: Gupta S, Termini J, Issac B, Guirado E, Stone G
    PLoS ONE, 2016-02-05;11(2):e0148929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Plasmacytoid dendritic cells and myeloid cells differently contribute to BAFF over-expression during primary HIV infection.
    Authors: Borhis G, Burelout C, Chaoul N, Smith N, Goujard C, Meyer L, Paul S, Saoudin H, Hosmalin A, Gilbert C, Herbeuval J, Richard Y
    AIDS, 2016-01-28;30(0):365-376.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFN-lambda) in cutaneous lupus erythematosus.
    Authors: Zahn S, Rehkamper C, Kummerer BM, Ferring-Schmidt S, Bieber T, Tuting T, Wenzel J
    J. Invest. Dermatol., 2010-08-19;131(1):133-40.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Neutralizing type I IFN antibodies trigger an IFN-like response in endothelial cells.
    Authors: Moll HP, Freudenthaler H, Zommer A
    J. Immunol., 2008-04-15;180(8):5250-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Virally stimulated plasmacytoid dendritic cells produce chemokines and induce migration of T and NK cells.
    Authors: Megjugorac NJ, Young HA, Amrute SB, Olshalsky SL, Fitzgerald-Bocarsly P
    J. Leukoc. Biol., 2004-01-23;75(3):504-14.
    Species: Human
    Sample Types:
    Applications: Flow Cytometry
  12. PRC2-mediated epigenetic suppression of type I IFN-STAT2 signaling impairs antitumor immunity in luminal breast cancer
    Authors: J Hong, JH Lee, Z Zhang, Y Wu, M Yang, Y Liao, R de la Rosa, J Scheirer, D Pechacek, N Zhang, Z Xu, T Curiel, X Tan, TH Huang, K Xu
    Cancer Research, 2022-12-16;0(0):.
  13. Autoantibodies against type I IFNs in patients with critical influenza pneumonia
    Authors: Qian Zhang, Andrés Pizzorno, Lisa Miorin, Paul Bastard, Adrian Gervais, Tom Le Voyer et al.
    Journal of Experimental Medicine

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