Home / HLA-DR / Human HLA-DR PE-conjugated Antibody

Human HLA-DR PE-conjugated Antibody

Catalog #: FAB4869P
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB4869P-100
FAB4869P-025
Detection of HLA‑DR in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HLA-DR PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HLA‑DR.
Source
Monoclonal Mouse IgG1 Clone # L203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
RPMI 8866 human lymphoblastoid cells
Accession # P01903
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of HLA-DR antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of HLA‑DR in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human HLA-DR PE-conjugated Monoclonal Antibody (Catalog # FAB4869P) or (B) isotype control antibody (Catalog # IC002P) and Mouse anti-Human CD19 APC-conjugated Monoclonal Antibody (Catalog # FAB4867A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Human HLA-DR by Flow Cytometry View Larger

Detection of Human HLA-DR by Flow Cytometry Costimulatory profile of mature DCs generated with a 3-day protocol. (A) Shown is the expression of specific surface molecules detected on DCs by flow cytometry in a representative patient sample generated with cocktail RP. (B) Comparison of the expression intensity of the costimulatory molecules CD80 and CD86 and the coinhibitory molecules CD273 and CD274 between HCs and AML patients. The mean fluorescence (MFI) of at least three individual experiments of HC and AML patients were compared using the different maturation cocktails C, CP, R, and RP. Image collected and cropped by CiteAb from the following publication (https://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-9-151), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: HLA-DR

HLA-DR is a transmembrane human major histocompatibility complex 2 (MHC II) family member and consists of a 34 kDa (alpha) subunit and one of several 28 kDa (beta) subunits. HLA-DR is expressed primarily by B cells and dendritic cells (DC), in which it binds peptides derived from internalized and processed antigenic proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD4+ T cells. This interaction is central to antigen specificity in the adaptive immune response. HLA-DR alleles, polymorphisms, and aberrant expression are linked to a variety of diseases including autoimmunity and cancer.

Long Name
Major Histocompatibility Complex Class II DR
Entrez Gene IDs
3122 (Human)
Alternate Names
FLJ51114; histocompatibility antigen HLA-DR alpha; HLA class II histocompatibility antigen, DR alpha chain; HLADR; HLA-DR; HLA-DRA; HLA-DRA1; major histocompatibility complex, class II, DR alpha; MHC cell surface glycoprotein; MHC class II antigen DRA; MHC Class II DR; MLRW

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Citations for Human HLA-DR PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Enhanced circulating ILC2s and MDSCs may contribute to ensure maintenance of Th2 predominant in patients with lung cancer
    Authors: Y Wu, Y Yan, Z Su, Q Bie, X Chen, PA Barnie, Q Guo, S Wang, H Xu
    Mol Med Rep, 2017-05-02;15(6):4374-4381.
  2. Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential
    Authors: Iris A. Otto, Paulina Nuñez Bernal, Margot Rikkers, Mattie H.P. van Rijen, Anneloes Mensinga, Moshe Kon et al.
    iScience
  3. Jiyuan oridonin A induces differentiation of acute myeloid leukemia cells including leukemic stem-like cells
    Authors: F Li, C Gao, X Li, J Wang, Y Zhao, Y Ke, Y Liu, HM Liu, Z Hu, L Wei, ZS Chen
    Frontiers in Pharmacology, 2022-09-05;13(0):1001552.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The Histone Deacetylase Inhibitor I1 Induces Differentiation of Acute Leukemia Cells With MLL Gene Rearrangements via Epigenetic Modification
    Authors: Jingfang Yao, Gentao Li, Zihui Cui, Peilei Chen, Jinhong Wang, Zhenbo Hu et al.
    Frontiers in Pharmacology
  5. The Histone Deacetylase Inhibitor I13 Induces Differentiation of M2, M3 and M5 Subtypes of Acute Myeloid Leukemia Cells and Leukemic Stem-Like Cells
    Authors: Xiangyu Ma, Mengjie Zhao, Zhuo-Xun Wu, Jingfang Yao, Lei Zhang, Jinhong Wang et al.
    Frontiers in Oncology
  6. Blood Monocyte Phenotype Fingerprint of Stable Coronary Artery Disease: A Cross-Sectional Substudy of SMARTool Clinical Trial
    Authors: S Sbrana, J Campolo, A Clemente, L Bastiani, A Cecchettin, E Ceccherini, C Caselli, D Neglia, O Parodi, D Chiappino, JM Smit, AJ Scholte, G Pelosi, S Rocchiccio
    Biomed Res Int, 2020-07-27;2020(0):8748934.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Effect of monocyte seeding density on dendritic cell generation in an automated perfusion-based culture system
    Authors: Andrew Kozbial, Lekhana Bhandary, Shashi K. Murthy
    Biochemical Engineering Journal
  8. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016-07-25;126(8):3130-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Circulating Myeloid-Derived Suppressor Cells Are Increased and Activated in Pulmonary Hypertension
    Authors: Michael E. Yeager, Cecilia M. Nguyen, Dmitry D. Belchenko, Kelley L. Colvin, Shinichi Takatsuki, D. Dunbar Ivy et al.
    Chest
  10. Effects of TLR agonists on maturation and function of 3-day dendritic cells from AML patients in complete remission.
    Authors: Beck B, Dorfel D, Lichtenegger FS, Geiger C, Lindner L, Merk M, Schendel DJ, Subklewe M
    J Transl Med, 2011-09-13;9(0):151.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Eosinophils induce DC maturation, regulating immunity.
    Authors: Lotfi R, Lotze MT
    J. Leukoc. Biol., 2007-11-08;83(3):456-60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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