Human HLA Class I Antibody

Catalog #: MAB11502 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11502-100
MAB11502-SP

Detection of Human HLA Class I by Western Blot.

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All HLA Class I Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HLA Class I Antibody Summary

Species Reactivity

Human

Specificity

Detects the human HLA-A protein by binding to its peptide region which includes Aspartic Acid (ASP) 338 as determined by ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1069511

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Membranes from human tonsillar lymphocytes

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

MDA-MB-231 human breast cancer cell line, Raji human Burkitt's lymphoma cell line and THP-1 human acute monocytic leukemia cell line

Flow Cytometry

2.5 µg/10⁶ cells

Detection of HLA Class I in PBMC by Flow Cytometry

Immunohistochemistry

3-25 µg/mL

Immersion fixed paraffin-embedded sections of human spleen

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human HLA Class I by Western Blot. Western Blot shows lysates of MDA‑MB‑231 human breast cancer cell line, Raji human Burkitt's lymphoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HLA Class I at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunohistochemistry

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Detection of HLA Class I in Human Spleen. HLA Class I was detected in immersion fixed paraffin-embedded sections of human spleen using Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) or the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Flow Cytometry

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Detection of HLA Class I in PBMC by Flow Cytometry PBMC were stained with Mouse Anti-Human CD19 APC‑conjugated Monoclonal Antibody (Catalog # FAB4867A) and either (A) Mouse Anti-Human HLA Class I Monoclonal Antibody (Catalog # MAB11502) or (B) isotype control antibody (Catalog # MAB004) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HLA Class I

HLA-A, B, and C are approximately 45 kDa transmembrane glycoproteins in the major histocompatibility complex 1 (MHC I) family. They contain three alpha domains in their extracellular regions. HLA molecules are expressed on nearly all nucleated cells in association with the 12 kDa beta 2-Microglobulin. This complex binds peptides derived from pathogenic cytosolic or extracellular proteins such as viral or microbial proteins. It presents these peptides on the cell surface for recognition by the T cell receptor on CD8+ cytotoxic T cells. The activated cytotoxic T cell then kills the presenting cell. Mismatched MHC I alleles between a host and a donor lead to transplant rejection.

References

Barnstable, C.J. et al. (1978) Cell 14:9.

Long Name

Class I Human Leukocyte Antigen

Entrez Gene IDs

3105 (Human)

Alternate Names

A-10 alpha chain; FLJ26655; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, A-28 alpha chain; HLA class I histocompatibility antigen, A-9 alpha chain; HLA Class I; HLAA; HLA-A; HLA-ABC; major histocompatibility complex, class I, A; MHC class I antigen A*1; MHC class I antigen A*11; MHC class I antigen A*80