Home / Histamine H1 R / Human Histamine H1 R Antibody

Human Histamine H1 R Antibody

Catalog #: MAB4726
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4726
MAB4726-SP
Detection of Histamine H1 R in PMA‑treated U937 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Histamine H1 R Antibody Summary

Species Reactivity
Human
Specificity
Detects human Histamine H1 R. Stains human Histamine H1 R transfectants but not irrelevant transfectants.
Source
Monoclonal Mouse IgG1 Clone # 480054
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Histamine H1 R
Met1-Ser487
Accession # AAH60802
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Histamine H1 R antibody in PMA-treated U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Histamine H1 R in PMA‑treated U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line treated with 50 ng/mL PMA for 24 hours was stained with Mouse Anti-Human Histamine H1 R Monoclonal Antibody (Catalog # MAB4726, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Flow Cytometry Detection of Histamine H1 R antibody in U937 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Histamine H1 R in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human Histamine H1 R Monoclonal Antibody (Catalog # MAB4726, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Histamine H1 R

Histamine H1 R (HRH1) is a 60 kDa, 487 aa G-protein coupled 7-transmembrane putative glycoprotein. It is implicated in the pathogenesis of asthma and is a major pharmaceutical target for antihistamines. It has been detected as a mixture of monomers and homodimers, with highest concentration in the placenta, followed by brain, lung (produced by airway smooth muscle, bronchial epithelium and macrophages) and other tissues. Extracellular domains of human HRH1 share 72% aa sequence identity with corresponding regions of mouse or rat HRH1.

Long Name
Histamine H1 Receptor
Entrez Gene IDs
3269 (Human); 15465 (Mouse); 24448 (Rat)
Alternate Names
H1R; H1-R; hisH1; Histamine H1 R; Histamine H1R; histamine receptor H1; HRH1; subclass H1

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Citations for Human Histamine H1 R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Evidence of promiscuous endothelial binding by P lasmodium falciparum ‐infected erythrocytes
    Authors: Claudia Esser, Anna Bachmann, Daniela Kuhn, Kathrin Schuldt, Birgit Förster, Meike Thiel et al.
    Cellular Microbiology
  2. Mycobacterium tuberculosis Utilizes Host Histamine Receptor H1 to Modulate Reactive Oxygen Species Production and Phagosome Maturation via the p38MAPK-NOX2 Axis
    Authors: S Mo, J Guo, T Ye, X Zhang, J Zeng, Y Xu, B Peng, Y Dai, W Xiao, P Zhang, G Deng, D Xu, X Long, Y Cai, X Chen
    MBio, 2022-08-24;0(0):e0200422.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Flow Cytometry

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