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Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay

Catalog #: FCSTM09
13 Citations 2 Reviews Datasheet / COA / SDS
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Human Luminex High Sensitivity Cytokine A Panel Standard Curves
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Summary Configure Your Assay Product Features Precision & Accuracy Data Examples Product Datasheets

Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay Summary

Panel Luminex Performance Human High Sensitivity Cytokine Panel A
Assay Type Magnetic bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Magnetic antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

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Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

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User Mixed
Please select at least one mixing option to see the kits.

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Split based on serum
Split based on plasma
Split based on cell culture supernates

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile up to analytes of your choice in one sample
  • Magnetic format allows for easier wash steps
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Kit Components
  • Premixed cocktail of antibody-coated Magnetic beads
  • Premixed cocktail of biotinylated detection antibody
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One flat-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 20 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across three different concentrations of analytes across 20 different serum assays.
  n=20 (10x2 levels) n=20
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
GM-CSF 4.2 13.3
IFN-gamma 4.5 12.2
IL-1 beta 5.2 12.8
IL-2 5.7 9.8
IL-4 5.3 10.3
IL-5 4.5 11.7
IL-6 4.9 9.7
IL-8 7.1 11.6
IL-10 5.9 10.9
IL-12 5.0 11.1
TNF-alpha 4.2 9.8
VEGF 7.0 13.4
 

Accuracy

Linearity - The data represents mean spiked linearity in serum matrix samples.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
  Linearity Spiked
Recovery
Analyte N Average % N Average %
GM-SCF 4 100 4 102
IFN-gamma 4 100 4 105
IL-1 beta 4 113 4 100
IL-2 4 104 4 96
IL-4 4 104 4 110
IL-5 4 116 4 85
IL-6 4 113 4 100
IL-8 4 123 4 86
IL-10 4 101 4 107
IL-12 4 100 4 103
TNF-alpha 4 112 4 86
VEGF 4 121 4 84
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Scientific Data

Multiplex Immunoassay Human Luminex High Sensitivity Cytokine A Panel Standard Curves View Larger

Human Luminex High Sensitivity Cytokine A Panel Standard Curves

Product Datasheets

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Citations for Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Flow Cytometric Analysis of Macrophages and Cytokines Profile in the Bronchoalveolar Lavage Fluid in Patients with Lung Cancer
    Authors: Kwiecie?, I;Rutkowska, E;Raniszewska, A;Rzeszotarska, A;Polubiec-Kownacka, M;Domaga?a-Kulawik, J;Korsak, J;Rzepecki, P;
    Cancers
    Species: Human
    Sample Types: BALF
  2. The Effect of Extremely Low-Frequency Electromagnetic Fields on Inflammation and Performance-Related Indices in Trained Athletes: A Double-Blinded Crossover Study
    Authors: Markus, I;Ohayon, E;Constantini, K;Geva-Kleinberger, K;Ibrahim, R;Ruban, A;Gepner, Y;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Predicting Lung Function Using Biomarkers in Alpha-1 Antitrypsin Deficiency
    Authors: Spittle, DA;Mansfield, A;Pye, A;Turner, AM;Newnham, M;
    Biomedicines
    Species: Human
    Sample Types: Serum
  4. Post-Traumatic Stress Response and Appendicitis in Children-Clinical Usefulness of Selected Biomarkers
    Authors: Sobczak, J;Burzyńska, M;Sikora, A;Wysocka, A;Karawani, J;Sikora, JP;
    Biomedicines
    Species: Human
    Sample Types: Serum
  5. H3K9 acetylation modification and TLR9 immune regulation mechanism in patients after anti-HBV treatment
    Authors: H Zhu, K Wang, W Du, H Cao, Q Zhong, S Yin, J Zhong, F Li
    Medicine, 2022-12-30;101(52):e32431.
    Species: Human
    Sample Types: Serum
  6. Evaluating Ocular Symptoms and Tear Film Cytokine Profiles in Symptomatic COVID-19 Patients
    Authors: A Nied?wied?, M Kawa, E Pius-Sadow, A Kuligowska, A Ziontkowsk, D Wrza?ek, M Parczewski, K Safranow, K Koz?owski, B Machali?sk, A Machali?sk
    Journal of Clinical Medicine, 2022-05-08;11(9):.
    Species: Human
    Sample Types: Tears
  7. Increased proinflammatory cytokines in tears correspond with conjunctival SARS-CoV-2 positivity in symptomatic COVID-19 patients
    Authors: A Nied?wied?, M Kawa, E Pius-Sadow, A Kuligowska, A Ziontkowsk, D Wrza?ek, MP Wi?cek, M Parczewski, A Ossowski, G Zieli?ska, K Safranow, K Koz?owski, B Machali?sk, A Machali?sk
    Scientific Reports, 2022-05-04;12(1):7225.
    Species: Human
    Sample Types: Tears
  8. Blood-based biomarkers of inflammation in amyotrophic lateral sclerosis
    Authors: KA Staats, DR Borchelt, MG Tansey, J Wymer
    Molecular Neurodegeneration, 2022-01-24;17(1):11.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Predictors of low ovarian reserve in cART-treated women living with HIV
    Authors: E Merlini, C Tincati, V Sacchi, M Augello, V Bono, ES Cannizzo, M Allegrini, L Gazzola, AD Monforte, AM Marconi, M Ravizza, G Marchetti
    Medicine, 2021-10-01;100(39):e27157.
    Species: Human
    Sample Types: Plasma
  10. Higher CD19+CD25+ Bregs are independently associated with better graft function in renal transplant recipients
    Authors: EH Ibrahim, MG Aly, G Opelz, C Morath, M Zeier, C Süsal, DM Sayed, E Hassan, N Ekpoom, V Daniel
    Bmc Nephrology, 2021-05-17;22(1):180.
    Species: Human
    Sample Types: Plasma
  11. Night shift schedule alters endogenous regulation of circulating cytokines
    Authors: PY Liu, MR Irwin, JM Krueger, S Gaddameedh, HPA Van Dongen
    Neurobiology of sleep and circadian rhythms, 2021-03-05;10(0):100063.
    Species: Human
    Sample Types: Serum
  12. Inflammaging markers characteristic of advanced age show similar levels with frailty and dependency
    Authors: A Alberro, A Iribarren-, M Sáenz-Cues, A Matheu, I Vergara, D Otaegui
    Scientific Reports, 2021-02-23;11(1):4358.
    Species: Human
    Sample Types: Plasma
  13. Longitudinal patterns of cytokine expression at the individual level in humans after laparoscopic sleeve gastrectomy
    Authors: U Trahtember, F Darawshe, R Elazary, I Ginsburg, M Beil, PV van Heerde, S Sviri
    J. Cell. Mol. Med., 2020-04-26;0(0):.
    Species: Human
    Sample Types: Serum

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Reviews for Human High Sensitivity Cytokine A Premixed Magnetic Luminex® Performance Assay

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Human High Sensitivity Cytokine A Premixed Magnetic Luminex Performance Assay
By Anonymous on 02/09/2022
Sample Tested: Plasma
Human High Sensitivity Cytokine A Premixed Magnetic Luminex Performance Assay
By Ping Yi on 09/13/2020
Sample Tested: Peripheral blood cells