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Human HGF Biotinylated Antibody

Catalog #: BAF294
9 Citations 1 Review Datasheet / COA / SDS
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BAF294
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Citations (9)
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Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HGF Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human (rh) HGF R, recombinant mouse HGF R, and rhMSP is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human HGF (Catalog # 294-HG)

Human HGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human HGF Antibody (Catalog # MAB694)

Standard: Recombinant Human HGF Protein (Catalog # 294-HG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity (1‑5). Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET (6, 7). HGF-dependent c-MET activation is implicated in the development of many human cancers (8). HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis (9, 10). HGF induces the upregulation of integrin  alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription (11). This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis (11, 12). HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions (13). In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake (14). HGF promotes the motility of cardiac stem cells in damaged myocardium (15).

References
  1. Karihaloo, A. et al. (2005) Nephron Exp. Nephrol. 100:e40.
  2. Hammond, D.E. et al. (2004) Curr. Top. Microbiol. Immunol. 286:21.
  3. Rosario, M. and W. Birchmeier (2004) Dev. Cell 7:3.
  4. Lesk, A.M. and W.D. Fordham (1996) J. Mol. Biol. 258:501.
  5. Nakamura, T. et al. (1989) Nature 342:440.
  6. Mizuno, K., et al. (1994) J. Biol. Chem. 269:1131.
  7. Gheradi, E. et al. (2003) Proc. Natl. Acad. Sci. 100:12039.
  8. Corso, S. et al. (2005) Trends Mol. Med. 11:284.
  9. Maeshima, A. et al. (2000) Kid. Int. 58:1511.
  10. Montesano, R. et al. (1991) Cell 67:901.
  11. Chiu, S-J. et al. (2002) J. Biomed. Sci. 9:261.
  12. Saelman, E.U.M. et al. (1995) J. Cell Sci. 108:3531.
  13. Tanaka, Y. et al. (2002) Biochem. Biophys. Res. Commun. 299:472.
  14. Mineo, R. et al. (1994) Endocrinology 145:4355.
  15. Urbanek, K. et al. (2005) Circ. Res. 97:663.
Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Citations for Human HGF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. In vitro differentiated human CD4+ T cells produce hepatocyte growth factor
    Authors: Shayne Lavondua Ford, Terkild Brink Buus, Claudia Nastasi, Carsten Geisler, Charlotte Menné Bonefeld, Niels Ødum et al.
    Frontiers in Immunology
  2. Cyanidin 3-Glucoside Induces Hepatocyte Growth Factor in Normal Human Dermal Fibroblasts through the Activation of beta 2-Adrenergic Receptor
    Authors: Annisa Krama, Natsu Tokura, Hiroko Isoda, Hideyuki Shigemori, Yusaku Miyamae
    ACS Omega
  3. A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF
    Authors: A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi
    Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295.
    Species: Rat
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC
  4. A receptor-antibody hybrid hampering MET-driven metastatic spread
    Authors: Chiara Modica, Cristina Basilico, Cristina Chiriaco, Nicla Borrelli, Paolo M. Comoglio, Elisa Vigna
    Journal of Experimental & Clinical Cancer Research
  5. Novel antibody reagents for characterization of drug- and tumor microenvironment-induced changes in epithelial-mesenchymal transition and cancer stem cells
    Authors: T Navas, TD Pfister, S Colantonio, A Aziz, L Dieckman, RG Saul, J Kaczmarczy, S Borgel, SY Alcoser, MG Hollingshe, YH Lee, DP Bottaro, T Hiltke, G Whiteley, N Takebe, RJ Kinders, RE Parchment, JE Tomaszewsk, JH Doroshow
    PLoS ONE, 2018-06-21;13(6):e0199361.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Development (Detection)
  6. Use of a Human Artificial Chromosome for Delivering Trophic Factors in a Rodent Model of Amyotrophic Lateral Sclerosis
    Authors: Yasuhiro Watanabe, Yasuhiro Kazuki, Kanako Kazuki, Mitsutaka Ebiki, Mami Nakanishi, Kazuomi Nakamura et al.
    Molecular Therapy - Nucleic Acids
  7. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  8. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-01-21;296(4):C922-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  9. Development and validation of sandwich ELISA microarrays with minimal assay interference.
    Authors: Gonzalez RM, Seurynck-Servoss SL, Crowley SA
    J. Proteome Res., 2008-04-19;7(6):2406-14.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Microarray Development

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Human HGF Biotinylated Antibody
By Anonymous on 11/16/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Human

A sandwich ELISA was made with MAB694 as the capture and BAF294 as the detection using 294-HG as the immunoassay calibrator.

ELISA Human HGF Biotinylated Antibody BAF294