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Human HGF Antibody

Catalog #: AB-294-NA
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AB-294-NA
IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human HGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse HGF is observed.
Source
Polyclonal Goat IgG
Purification
Protein A or G purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF (R&D Systems, Catalog # 294-HG)
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human HGF (Catalog # 294-HG)
Neutralization
Measured by its ability to neutralize HGF-induced IL-11 secretion in the Saos‑2 human osteosarcoma cell line. Hjertner, O. et al. (1999) Blood 94:3883. The Neutralization Dose (ND50) is typically <2 μg/mL in the presence of 3 ng/mL Recombinant Human HGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. View Larger

IL-11 Secretion Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HGN) induces IL-11 secretion in the Saos-2 human osteosarcoma cell line. The dose-dependent response (orange line) is measured by the Human IL-11 Duoset (Catalog # DY218). Under these conditions, IL-11 secretion elicited by HGF is neutralized (green line) by increasing concentrations of Goat Anti-Human HGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-294-NA). The ND50 is typically <2 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 1 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE-like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Human HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide‑linked HGF with a 60 kDa alpha  and 30 kDa beta chain. Alternate splicing generates human HGF isoforms that lack the proteinase-like domain and different numbers of the Kringle domains. Human HGF shares 91%‑94% aa sequence identity with bovine, canine, feline, mouse, and rat HGF. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF-dependent c-MET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin alpha 2 beta 1 in epithelial cells by a selective increase in alpha 2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions. In the thyroid, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSH-stimulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium.

Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Citations for Human HGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF
    Authors: A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi
    Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  2. Targeting hepatocyte growth factor in epithelial–stromal interactions in an in vitro experimental model of human periodontitis
    Authors: Yoko Yamaguchi, Akira Saito, Masafumi Horie, Akira Aoki, Patrick Micke, Mitsuhiro Ohshima et al.
    Odontology
  3. Acidic fibroblast growth factor underlies microenvironmental regulation of MYC in pancreatic cancer
    Authors: Sohinee Bhattacharyya, Chet Oon, Aayush Kothari, Wesley Horton, Jason Link, Rosalie C. Sears et al.
    Journal of Experimental Medicine
  4. Efficient blockade of locally reciprocated tumor-macrophage signaling using a TAM-avid nanotherapy
    Authors: SJ Wang, R Li, TSC Ng, G Luthria, MJ Oudin, M Prytyskach, RH Kohler, R Weissleder, DA Lauffenbur, MA Miller
    Sci Adv, 2020-05-22;6(21):eaaz8521.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Hepatocyte growth factor produced in lung fibroblasts enhances non-small cell lung cancer cell survival and tumor progression
    Authors: N Kanaji, M Yokohira, Y Nakano-Nar, N Watanabe, K Imaida, N Kadowaki, S Bandoh
    Respir. Res., 2017-06-15;18(1):118.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Heparin-binding epidermal growth factor-like growth factor and hepatocyte growth factor inhibit cholestatic liver injury in mice through different mechanisms
    Authors: Kouichi Sakamoto, Ngin Cin Khai, Yuqing Wang, Rie Irie, Hideo Takamatsu, Hiroshi Matsufuji et al.
    International Journal of Molecular Medicine
  7. c-Met-mediated endothelial plasticity drives aberrant vascularization and chemoresistance in glioblastoma
    Authors: M Huang, T Liu, P Ma, RA Mitteer, Z Zhang, HJ Kim, E Yeo, D Zhang, P Cai, C Li, L Zhang, B Zhao, L Roccogrand, DM O'Rourke, N Dahmane, Y Gong, C Koumenis, Y Fan
    J Clin Invest, 2016-04-04;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Identification of drugs as single agents or in combination to prevent carcinoma dissemination in a microfluidic 3D environment
    Authors: Jing Bai, Ting-Yuan Tu, Choong Kim, Jean Paul Thiery, Roger D. Kamm
    Oncotarget
  9. HGF Mediates the Anti-inflammatory Effects of PRP on Injured Tendons
    Authors: Jianying Zhang, Kellie K. Middleton, Freddie H. Fu, Hee-Jeong Im, James H-C. Wang
    PLoS ONE
  10. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009-01-21;296(4):C922-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development

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