Human HB-EGF APC-conjugated Antibody Summary
rhHRG-beta, rhEGF, or rhTGF-alpha.
Asp63-Leu148
Accession # Q53H93
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of HB‑EGF in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Mouse Anti-Human HB-EGF APC-conjugated Monoclonal Antibody (Catalog # IC259A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: HB-EGF
HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogenous O‑glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.
HB-EGF is a member of the EGF family of mitogens which also include Transforming Growth Factor-alpha (TGF-alpha ), Amphiregulin (AR), rat Schwanoma-derived Growth Factor (SDGF), Vaccinia Growth Factor (VGF), and the various ligands for the HER2/ErbB2/Neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha, and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.
Product Datasheets
Citations for Human HB-EGF APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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CD206+ tumor-associated macrophages promote proliferation and invasion in oral squamous cell carcinoma via EGF production
Authors: ASMR Haque, M Moriyama, K Kubota, N Ishiguro, M Sakamoto, A Chinju, K Mochizuki, T Sakamoto, N Kaneko, R Munemura, T Maehara, A Tanaka, JN Hayashida, S Kawano, T Kiyoshima, S Nakamura
Sci Rep, 2019-10-10;9(1):14611.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Long-Term Depletion of Conventional Dendritic Cells Cannot Be Maintained in an Atherosclerotic Zbtb46-DTR Mouse Model
Authors: Miche Rombouts, Nathalie Cools, Mandy O. J. Grootaert, Flore de Bakker, Ilse Van Brussel, An Wouters et al.
PLOS ONE
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Evaluation of the BDCA2-DTR Transgenic Mouse Model in Chronic and Acute Inflammation.
Authors: Mandl M, Drechsler M, Jansen Y, Neideck C, Noels H, Faussner A, Soehnlein O, Weber C, Doring Y
PLoS ONE, 2015-08-07;10(8):e0134176.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
FAQs
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Why was intracellular staining performed instead of surface staining with IC259A?
Staining with IC259A worked optimally for our samples when intracellular Flow staining protocol was used. The antibody could be tried with both surface and intracellular staining protocols whan a different sample type is used.
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