Human GPR12 PE-conjugated Antibody Summary
Accession # P47775
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of GPR12 in HEK293 Human Cell Line Transfected with Human GPR12 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human GPR12 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human GPR12 PE-conjugated Monoclonal Antibody (Catalog # FAB6738P). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: GPR12
GPR12 is a 7-transmembrane receptor for sphingosylphosphorylcholine. Human GPR12 and the related GPR3 and GPR6 are all highly expressed on neurons of the central nervous system, while GPR12 is particularly expressed in the limbic system. GPR12 engagement promotes proliferation and maturation of postmitotic neurons through an inhibitory G protein and cAMP signaling pathway. Human GPR12 cDNA encodes 334 amino acids (aa), 89 of which are extracellular; these share 86% aa identity with mouse and rat GPR12.
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