Human GHSR Antibody

Catalog #: MAB8370 Datasheet / COA / SDS

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MAB8370

Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry.

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Human GHSR Antibody Summary

Species Reactivity

Human

Specificity

Detects GHSR on transfectants by flow cytometry.

Source

Monoclonal Mouse IgG₁ Clone # 502430

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human GHSR
Accession # NP_940799

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GHSR and eGFP was stained with either (A) Mouse Anti-Human GHSR Monoclonal Antibody (Catalog # MAB8370) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHSR

GHSR is a member of the G protein-coupled receptor family that may play a role in energy homeostasis and regulation of body weight. Two conserved transcript variants for GHSR are expressed in several tissues. This transcript, 1a, excises an intron and encodes the functional protein. GHSR is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. Mutations in GHSR are associated with autosomal idiopathic short stature.

Long Name

Growth Hormone Secretagogue Receptor

Entrez Gene IDs

2693 (Human); 208188 (Mouse); 84022 (Rat)

Alternate Names

GH-releasing peptide receptor; Ghrelin receptor; GHRP; GHSR; GHS-R; growth hormone secretagogue receptor type 1; growth hormone secretagogue receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

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