Human GHSR Antibody

Catalog # Availability Size / Price Qty
MAB8370
Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry.
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Human GHSR Antibody Summary

Species Reactivity
Human
Specificity
Detects GHSR on transfectants by flow cytometry.
Source
Monoclonal Mouse IgG1 Clone # 502430
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human GHSR
Accession # NP_940799
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of GHSR antibody in HEK293 Human Cell Line Transfected with Human GHSR and eGFP antibody by Flow Cytometry. View Larger

Detection of GHSR in HEK293 Human Cell Line Transfected with Human GHSR and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human GHSR and eGFP was stained with either (A) Mouse Anti-Human GHSR Monoclonal Antibody (Catalog # MAB8370) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GHSR

GHSR is a member of the G protein-coupled receptor family that may play a role in energy homeostasis and regulation of body weight. Two conserved transcript variants for GHSR are expressed in several tissues. This transcript, 1a, excises an intron and encodes the functional protein. GHSR is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. Mutations in GHSR are associated with autosomal idiopathic short stature.

Long Name
Growth Hormone Secretagogue Receptor
Entrez Gene IDs
2693 (Human); 208188 (Mouse); 84022 (Rat)
Alternate Names
GH-releasing peptide receptor; Ghrelin receptor; GHRP; GHSR; GHS-R; growth hormone secretagogue receptor type 1; growth hormone secretagogue receptor

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