Human GATA-2 PE-conjugated Antibody

Catalog #: IC2046P
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IC2046P

Detection of GATA‑2 in TF‑1 Human Cell Line by Flow Cytometry.

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All GATA-2 Antibodies

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Human GATA-2 PE-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GATA-2. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-1, rhGATA-5 and rhGATA-6.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human GATA-2
Ala15-Thr279
Accession # P23769

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of GATA-2 antibody in TF-1 Human Cell Line antibody by Flow Cytometry.

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Detection of GATA‑2 in TF‑1 Human Cell Line by Flow Cytometry. TF-1 human erythroleukemic cell line was stained with Goat Anti-Human GATA-2 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2046P, filled histogram) or control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: GATA-2

GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).

References

Tsai, S.F. et al. (1989) Nature 339:446.
Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

Entrez Gene IDs

2624 (Human)

Alternate Names

endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA2; GATA-2; GATA-binding protein 2NFE1B; MGC2306

Product Datasheets

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Citation for Human GATA-2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1