Summary
Precision
Recovery
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (10 uL)
Sensitivity
0.161 ng/mL
Assay Range
0.4 - 25 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Natural and recombinant human Galectin-3BP/MAC-2BP
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Human Galectin-3BP/MAC-2BP Immunoassay is a 4.5 hour solid phase ELISA
designed to measure Galectin-3BP in cell culture supernates, serum, plasma,
saliva, and urine. It contains NS0-expressed recombinant
human Galectin-3BP and antibodies raised against the recombinant factor. This
immunoassay has been shown to accurately quantitate recombinant human Galectin-3BP. Results obtained using natural human Galectin-3BP showed linear curves
that were parallel to the standard curves obtained using the Quantikine
kit standards. These results indicate that this kit can be used to
determine relative mass values for natural human Galectin-3BP.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of a known concentration were tested in twenty separate
assays to assess inter-assay precision. Assays were performed by at
least three technicians using two lots of kit components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 32 | 32 |
| Mean (pg/mL) | 0.743 | 2.94 | 8.27 | 0.778 | 3.2 | 9.1 |
| Standard Deviation | 0.027 | 0.08 | 0.244 | 0.078 | 0.225 | 0.434 |
| CV% | 3.6 | 2.7 | 3 | 10 | 7 | 4.8 |
Recovery
The recovery of human Galectin-3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=8) | 99 | 95-101 |
| Saliva (n=4) | 91 | 82-101 |
| Urine (n=4) | 104 | 98-111 |
Linearity
To assess the linearity of the assay, samples containing high
concentrations of Galectin-3BP were serially diluted with the appropriate
Calibrator Diluent to produce samples with values within the dynamic
range of the assay. Samples were diluted prior to assay.
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Galectin-3BP/MAC-2BP
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
Long Name:
Galectin-3 Binding Protein
Entrez Gene IDs:
3959 (Human)
Alternate Names:
90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; Galectin3BP; Galectin-3BP; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
200 µL Conjugate
200 µL Substrate Solution
50 µL Stop Solution
Citations for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Glycosphingolipid Levels in Urine Extracellular Vesicles Enhance Prediction of Therapeutic Response in Lupus Nephritis
Authors: B Troyer, J Rodgers, BJ Wolf, JC Oates, RR Drake, TK Nowling
Metabolites, 2022-02-01;12(2):.
Species: Human
Sample Types: Urine
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SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care
Authors: C Gutmann, K Takov, SA Burnap, B Singh, H Ali, K Theofilato, E Reed, M Hasman, A Nabeebaccu, M Fish, MJ McPhail, K O'Gallaghe, LE Schmidt, C Cassel, M Rienks, X Yin, G Auzinger, S Napoli, SF Mujib, F Trovato, B Sanderson, B Merrick, U Niazi, M Saqi, K Dimitrakop, R Fernández-, S Braun, R Kronstein-, KJ Doores, JD Edgeworth, AM Shah, SR Bornstein, T Tonn, AC Hayday, M Giacca, M Shankar-Ha, M Mayr
Nature Communications, 2021-06-07;12(1):3406.
Species: Human
Sample Types: Plasma
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Investigating LGALS3BP/90?K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
Authors: J Costa, A Pronto-Lab, S Pinto, M Gromicho, S Bonucci, E Tranfield, C Correia, BM Alexandre, M de Carvalh
Sci Rep, 2020-03-27;10(1):5649.
Species: Human
Sample Types: CSF
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Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017-03-20;0(0):.
Species: Human
Sample Types: Plasma
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Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
Sci Rep, 2017-01-23;7(0):40994.
Species: Human
Sample Types: Serum
FAQs
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Reviews for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
Average Rating: 4.7 (Based on 3 Reviews)
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Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
By Anonymous
on 09/13/2020
Sample Tested: Serum
Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
By Anonymous
on 10/08/2018
Sample Tested: HASMC human aortic smooth muscle cells
Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
By Christian Cassel
on 08/29/2018
Sample Tested: EDTA Plasma
