Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 32 | 32 | 32 |
Mean (pg/mL) | 0.743 | 2.94 | 8.27 | 0.778 | 3.2 | 9.1 |
Standard Deviation | 0.027 | 0.08 | 0.244 | 0.078 | 0.225 | 0.434 |
CV% | 3.6 | 2.7 | 3 | 10 | 7 | 4.8 |
Recovery
The recovery of human Galectin-3BP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
Sample Type | Average % Recovery | Range % |
---|---|---|
Cell Culture Media (n=8) | 99 | 95-101 |
Saliva (n=4) | 91 | 82-101 |
Urine (n=4) | 104 | 98-111 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: Galectin-3BP/MAC-2BP
The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one or two carbohydrate recognition domains (CRR) which mediate recognition of N-acetyl-lactosamine-containing glycoproteins. Some galectins exist in multiple isoforms due to alternative splicing. Individual galectins differ in their tissue distribution and in their carbohydrate-binding specificities.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Galectin-3BP/MAC-2BP Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Glycosphingolipid Levels in Urine Extracellular Vesicles Enhance Prediction of Therapeutic Response in Lupus Nephritis
Authors: B Troyer, J Rodgers, BJ Wolf, JC Oates, RR Drake, TK Nowling
Metabolites, 2022-02-01;12(2):.
Species: Human
Sample Types: Urine
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SARS-CoV-2 RNAemia and proteomic trajectories inform prognostication in COVID-19 patients admitted to intensive care
Authors: C Gutmann, K Takov, SA Burnap, B Singh, H Ali, K Theofilato, E Reed, M Hasman, A Nabeebaccu, M Fish, MJ McPhail, K O'Gallaghe, LE Schmidt, C Cassel, M Rienks, X Yin, G Auzinger, S Napoli, SF Mujib, F Trovato, B Sanderson, B Merrick, U Niazi, M Saqi, K Dimitrakop, R Fernández-, S Braun, R Kronstein-, KJ Doores, JD Edgeworth, AM Shah, SR Bornstein, T Tonn, AC Hayday, M Giacca, M Shankar-Ha, M Mayr
Nature Communications, 2021-06-07;12(1):3406.
Species: Human
Sample Types: Plasma
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Investigating LGALS3BP/90?K glycoprotein in the cerebrospinal fluid of patients with neurological diseases
Authors: J Costa, A Pronto-Lab, S Pinto, M Gromicho, S Bonucci, E Tranfield, C Correia, BM Alexandre, M de Carvalh
Sci Rep, 2020-03-27;10(1):5649.
Species: Human
Sample Types: CSF
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Extracellular matrix proteomics identifies molecular signature of symptomatic carotid plaques
Authors: SR Langley, K Willeit, A Didangelos, LP Matic, P Skroblin, J Barallobre, M Lengquist, G Rungger, A Kapustin, L Kedenko, C Molenaar, R Lu, T Barwari, G Suna, X Yin, B Iglseder, B Paulweber, P Willeit, J Shalhoub, G Pasterkamp, AH Davies, C Monaco, U Hedin, CM Shanahan, J Willeit, S Kiechl, M Mayr
J. Clin. Invest, 2017-03-20;0(0):.
Species: Human
Sample Types: Plasma
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Serum levels of galectin-1, galectin-3, and galectin-9 are associated with large artery atherosclerotic stroke
Authors: XW He, WL Li, C Li, P Liu, YG Shen, M Zhu, XP Jin
Sci Rep, 2017-01-23;7(0):40994.
Species: Human
Sample Types: Serum
FAQs
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