Human GAD1/GAD67 Antibody

Catalog #: MAB2086 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2086
MAB2086-SP

All GAD1/GAD67 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GAD1/GAD67 Antibody Summary

Species Reactivity

Human

Specificity

Detects human GAD1/GAD67 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 252910

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human GAD1/GAD67

Immunocytochemistry

8-25 µg/mL

Immersion fixed paraffin-embedded section of human spinal cord

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GAD1/GAD67

GAD1, also known as GAD67, is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions and putative paracrine hormone in pancreatic islets. GAD occurs as two molecular forms, 65 kDa and 67 kDa, that are encoded by separate genes and share 65% amino acid identity. GAD1 is highly conserved and is expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary (1).

References