Summary
Precision
Data Examples
Product Datasheets
Preparation and Storage
Background
Related Research Areas
Human Free IGF-I/IGF-1 ELISA Kit Summary
Assay Length
1.75 hours
Sample Type & Volume Required Per Well
EDTA Plasma (50 uL)
Sensitivity
0.006 ng/mL
Assay Range
0.1 - 6 ng/mL (EDTA Plasma)
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Free IGF-I enzyme linked immunosorbent assay (ELISA) is a 1.75 hour solid-phase ELISA designed to measure human Free IGF-I in EDTA Plasma. It contains E. coli-expressed recombinant human IGF-I and has been shown to accurately quantitate the recombinant factor.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in thirty-two separate assays to assess inter-assay precision
EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 32 | 32 | 32 |
| Mean (ng/mL) | 0.669 | 2.04 | 4.53 | 0.677 | 2.03 | 4.29 |
| Standard Deviation | 0.014 | 0.041 | 0.135 | 0.038 | 0.088 | 0.199 |
| CV% | 2.1 | 2 | 3 | 5.6 | 4.3 | 4.6 |
Scientific Data
Product Datasheets
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-I/IGF-1
IGF-1 (previously called somatomedin C) is a 7.6 kDa, 70 amino acid (aa) polypeptide with three internal disulfide bonds. The sequence of human IGF-1 is identical to that of bovine and porcine IGF-1, and it is 70% identical to human IGF-II. IGF-1 is a single-chain molecule with about 50% identity to the sequences of the A- and B-chains of human insulin.
While IGF-1 has very complex functions, it appears largely
to mediate the actions of growth hormone. Thus, IGF-1 is important in prenatal
development, growth to adulthood and metabolic control. It induces amino acid
uptake, protein synthesis and glucose utilization. It is an important mitogen
and regulator of the cell cycle and apoptosis. IGF-1 is produced primarily by
hepatocytes, serving an endocrine function. It is also produced by many other
cells, where it may act in an autocrine or paracrine manner. Serum levels of
IGF-1 have been reported to increase from birth to puberty, followed by a slow
decline through adulthood.
There are two receptors for the IGFs; type I IGF receptor,
which signals through a tyrosine kinase, and type II IGF receptor, which is
identical to the mannose-6-phosphate receptor and may not signal. The action of
IGF on its receptors is very complex, with control by at least six IGF-binding
proteins (IGFBP-1 through IGFBP-6). IGFBP-3 binds over 90% of the total IGF in
serum in a complex of IGF, IGFBP and an acid-labile subunit. This ternary
complex greatly stabilizes IGF in the circulation, changing the half life from
minutes to hours. In addition, IGFBPs modulate the action of IGF on the
membrane receptors. Adding to the complexity is a family of proteases that act
on IGFBPs, modifying their affinity for IGF or completely eliminating the
IGFBPs. The interactions of IGF, IGFBP, IGFBP proteases, and IGF receptors are
referred to as the IGF axis. The IGF axis affects many primary physiological
and pathological processes, including development, growth, metabolic regulation,
tumorigenesis, atherosclerosis and angiogenesis.
Long Name:
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs:
3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names:
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.
Citations for Human Free IGF-I/IGF-1 ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Trio, a novel bovine high fecundity allele: III. Acquisition of dominance and ovulatory capacity at a smaller follicle size
Authors: A García-Gue, AMO Canavessi, PLJM Jr, MA Mezera, R Sartori, BW Kirkpatric, MC Wiltbank
Biol. Reprod., 2018-03-01;0(0):.
Species: Bovine
Sample Types: Follicular Fluid
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Detection of Placental Proteomes at Different Uterine Positions in Large White and Meishan Gilts on Gestational Day 90
PLoS ONE, 2016-12-09;11(12):e0167799.
Species: Porcine
Sample Types: Serum
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IGFBP-2 inhibits adipogenesis and lipogenesis in human visceral, but not subcutaneous, adipocytes.
Authors: Yau S, Russo V, Clarke I, Dunshea F, Werther G, Sabin M
Int J Obes (Lond), 2014-11-05;39(5):770-81.
Species: Human
Sample Types: Cell Culture Supernates
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Human Free IGF-I/IGF-1 ELISA Kit
By Anonymous
on 02/25/2023
Sample Tested: Serum and Plasma
