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Human FoxH1 Antibody

Catalog #: AF4248
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4248
AF4248-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FoxH1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FoxH1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human FoxB2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxH1
Ser164-Ser272
Accession # O75593
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human FoxH1
Immunocytochemistry
5-15 µg/mL
Immersion fixed BG01V human embryonic stem cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxH1

FoxH1 (also known as FAST-1) is a 39 kDa, class 2, subfamily H member of the FOX (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22‑30), one FKH (fork head) DNA-binding domain (aa 32‑128) and a SMAD-interaction region (aa 273‑354). Over aa 164‑272, human FOXH1 is 55% identical to mouse FoxH1.

Long Name
Forkhead Box J1
Entrez Gene IDs
8928 (Human)
Alternate Names
FAST1; Fast-1; FAST1forkhead box protein H1; FAST2; Fast-2; Forkhead activin signal transducer 1; Forkhead activin signal transducer 2; forkhead activin signal transducer-1; forkhead box H1; FoxH1; hFAST-1; TGF-beta/activin signal transducer

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Citations for Human FoxH1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Antagonism of Nodal signaling by BMP/Smad5 prevents ectopic primitive streak formation in the mouse amnion.
    Authors: Pereira P, Dobreva M, Maas E, Cornelis F, Moya I, Umans L, Verfaillie C, Camus A, de Sousa Lopes S, Huylebroeck D, Zwijsen A
    Development, 2012-09-01;139(18):3343-54.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Chromatin and transcriptional signatures for Nodal signaling during endoderm formation in hESCs.
    Authors: Kim SW, Yoon SJ, Chuong E, Oyolu C, Wills AE, Gupta R, Baker J
    Dev. Biol., 2011-06-29;357(2):492-504.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP

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