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Human FGF-BP Antibody

Catalog #: MAB1593
8 Citations Datasheet / COA / SDS
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MAB1593
MAB1593-SP
Detection of Human FGF-BP by Western Blot
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Citations (8)
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Human FGF-BP Antibody Summary

Species Reactivity
Human
Specificity
Detects human FGF-BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant rat FGF-BP is observed.
Source
Monoclonal Mouse IgG1 Clone # 196519
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FGF-BP
Val35-Cys234
Accession # Q14512
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human FGF-BP (Catalog # 1593-FB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human FGF-BP by Western Blot View Larger

Detection of Human FGF-BP by Western Blot miR-217 targets KLF5 by binding to its 3’UTR.A. miR-217 decreased the KLF5, FGF-BP and Cyclin D1 protein levels in HCC1806 and HCC1937 TNBC cells. KLF5, FGF-BP and Cyclin D1 protein levels were detected by using WB. beta -actin was used as the loading control. B. The putative wild type binding sites of miR-217 on KLF5 3’UTR and its mutants. C. miR-217 mimics significantly inhibits the KLF5 3’UTR luciferase reporter activity through the second putative binding site. HEK293T cells were transfected with miR-217 mimics and pMIR-KLF5 3’-UTR or miR-217 binding sites mutated pMIR-KLF5 3’-UTR reporters (mut1, mut2 or mut1,2) together with the pCMV-Renilla control. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0176395), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FGF-BP

Fibroblast growth factor binding protein (FGF‑BP), also known as HBp17, is a secreted glycoprotein that increases the bioavailability of FGFs (1). Mature FGF-BP is a 34 kDa, 211 amino acid (aa) O-glycosylated protein with five conserved intrachain disulfide bonds (2‑4). FGF-BP contains a heparin-binding domain (aa 110‑143) and a distinct FGF-binding region (aa 193‑243) (5). Mature human FGF‑BP shares 59% and 54% aa sequence identity with mouse and rat FGF-BP, respectively. FGF-BP is expressed throughout development and in adult squamous epithelium (2, 6). It is upregulated in injured skin, renal tubular epithelium, and spinal nerves as well as in carcinomas of the skin, colon, and pancreas (3, 7‑10). FGF-BP binds FGF-1, -2, -7, -10, and -22 which are secreted and sequestered in the extracellular matrix (ECM) (7, 11). The association of FGF-BP with heparan sulfate proteoglycans (HSPG) weakens HSPG attachment of FGFs and promotes their release (2, 8, 12, 13). FGF‑BP enhances the mitogenic effects of FGFs, thereby contributing to epithelial, endothelial, and neuronal tissue repair, angiogenesis, and tumor growth (7‑9, 11, 14, 15).

References
  1. Abuharbeid, S. et al. (2006) Int. J. Biochem. Cell Biol. 38:1463.
  2. Wu, D. et al. (1991) J. Biol. Chem. 266:16778.
  3. Tassi, E. et al. (2006) Cancer Res. 66:1191.
  4. Lametsch, R. et al. (2000) J. Biol. Chem. 275:19469.
  5. Xie, B. et al. (2006) J. Biol. Chem. 281:1137.
  6. Aigner, A. et al. (2002) Histochem. Cell Biol. 117:1.
  7. Beer, H-D. et al. (2005) Oncogene 24:5269.
  8. Ray, P.E. et al. (2006) Am. J. Physiol. Regul. Integr. Comp. Physiol. 290:R105.
  9. Tassi, E. et al. (2007) Am. J. Physiol. Regul. Integr. Comp. Physiol. 293:R775.
  10. Kurtz, A. et al. (2004) Neoplasia 6:595.
  11. Tassi, E. et al. (2001) J. Biol. Chem. 276:40247.
  12. Mongiat, M. et al. (2001) J. Biol. Chem. 276:10263.
  13. Kurtz, A. et al. (1997) Oncogene 14:2671.
  14. Aigner, A. et al. (2001) Int. J. Cancer 92:510.
  15. Czubayko, F. et al. (1997) Nat. Med. 3:1137.
Long Name
Fibroblast Growth Factor Binding Protein
Entrez Gene IDs
9982 (Human); 14181 (Mouse); 64535 (Rat)
Alternate Names
17 kDa heparin-binding growth factor-binding protein; FGFBP; FGF-BP; FGFBP1; FGF-BP1; FGFBP-1; FGFBPFGF-binding protein 1; fibroblast growth factor binding protein 1; fibroblast growth factor-binding protein 1; HBp17; HBP1717 kDa HBGF-binding protein; heparin-binding growth factor binding protein

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Citations for Human FGF-BP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. BRD4-specific PROTAC inhibits basal-like breast cancer partially through downregulating KLF5 expression
    Authors: Kong, Y;Lan, T;Wang, L;Gong, C;Lv, W;Zhang, H;Zhou, C;Sun, X;Liu, W;Huang, H;Weng, X;Cai, C;Peng, W;Zhang, M;Jiang, D;Yang, C;Liu, X;Rao, Y;Chen, C;
    Oncogene
    Species: Human
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Histone Deacetylase Inhibitors (HDACi) Promote KLF5 Ubiquitination and Degradation in Basal-like Breast Cancer
    Authors: Y Kong, W Ren, H Fang, NA Shah, Y Shi, D You, C Zhou, D Jiang, C Yang, H Liang, W Liu, L Wang, W Gan, X Wan, F Li, Z Li, C Chen, N Xie
    International journal of biological sciences, 2022-02-28;18(5):2104-2115.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Poly-L-arginine promotes asthma angiogenesis through induction of FGFBP1 in airway epithelial cells via activation of the mTORC1-STAT3 pathway
    Authors: X Chen, M Miao, M Zhou, J Chen, D Li, L Zhang, A Sun, M Guan, Z Wang, P Liu, S Zhang, X Zha, X Fan
    Cell Death & Disease, 2021-08-02;12(8):761.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  4. USP3 promotes breast cancer cell proliferation by deubiquitinating KLF5
    Authors: Y Wu, J Qin, F Li, C Yang, Z Li, Z Zhou, H Zhang, Y Li, X Wang, R Liu, Q Tao, W Chen, C Chen
    J. Biol. Chem., 2019-10-17;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. miR-217 inhibits triple-negative breast cancer cell growth, migration, and invasion through targeting KLF5
    Authors: W Zhou, F Song, Q Wu, R Liu, L Wang, C Liu, Y Peng, S Mao, J Feng, C Chen
    PLoS ONE, 2017-04-24;12(4):e0176395.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. Metformin suppresses triple-negative breast cancer stem cells by targeting KLF5 for degradation
    Authors: Peiguo Shi, Wenjing Liu, Tala, Haixia Wang, Fubing Li, Hailin Zhang et al.
    Cell Discovery
  7. YAP promotes breast cell proliferation and survival partially through stabilizing the KLF5 transcription factor.
    Authors: Zhi X, Zhao D, Zhou Z, Liu R, Chen C
    Am. J. Pathol., 2012-06-01;180(6):2452-61.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Gene expression signatures for tumor progression, tumor subtype, and tumor thickness in laser-microdissected melanoma tissues.
    Authors: Jaeger J, Koczan D, Thiesen HJ, Ibrahim SM, Gross G, Spang R, Kunz M
    Clin. Cancer Res., 2007-02-01;13(3):806-15.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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