Human FCRL5/FCRL3 PE-conjugated Antibody Summary
Gln16-Arg844
Accession # AAI01067
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of FCRL5/FCRL3 in CD19+Human Lymphocytes by Flow Cytometry. CD19+human lymphocytes were stained with Mouse Anti-Human FCRL5/FCRL3 PE-conjugated Monoclonal Antibody (Catalog # FAB20871P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FCRL5/FCRL3
Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one Immunotyrosine Activation Motif (ITAM) and two Immunotyrosine Inhibitory Motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5-7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up-regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up-regulated on tumor cells in some types of B cell malignancies (6, 10-12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).
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- Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
- Hatzivassiliou, G. et al. (2001) Immunity 14:277.
- SwissProt # Q96RD9.
- Miller, I. et al. (2002) Blood 99:2662.
- Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
- Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
- Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
- Mohan, J. et al. (2006) Blood 107:4433.
- Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
- Ise, T. et al. (2007) Leukemia 21:169.
- Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
- Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
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