Home / FCRL5/FCRL3 / Human FCRL5/FCRL3 Antibody

Human FCRL5/FCRL3 Antibody

Catalog #: MAB20871 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB20871
MAB20871-SP
Detection of FCRL5/FCRL3 in Human CD19+Lymphocytes by Flow Cytometry.
1 Image
Product Details
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human FCRL5/FCRL3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human FCRL5/FCRL3 in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) FCRL3 is observed and no cross-reactivity with rhFCRL1, 2, or 4 is observed.
Source
Monoclonal Mouse IgG2b Clone # 307307
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FCRL5/FCRL3 antibody in Human CD19<SUP>+</SUP>Lymphocytes antibody by Flow Cytometry. View Larger

Detection of FCRL5/FCRL3 in Human CD19+Lymphocytes by Flow Cytometry. Human blood-derived CD19+lymphocytes were stained with Mouse Anti-Human FCRL5/FCRL3 Monoclonal Antibody (Catalog # MAB20871, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: FCRL5/FCRL3

Fc Receptor-Like 5 (FCRL5), also known as FcRH5, IRTA2, and CD307, is a 120 kDa protein with sequence homology to classical Fc receptors. The type 1 transmembrane FCRL proteins contain from three to nine immunoglobulin-like domains. They are differentially expressed within the B cell lineage and can either promote or inhibit B cell proliferation and activation (1, 2). According to R&D Systems testing, FCRL5 binds to purified human IgG with high affinity. Mature human FCRL5 consists of a 836 amino acid (aa) extracellular domain (ECD) with nine Ig-like domains, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain with one immunotyrosine activation motif (ITAM) and two immunotyrosine inhibitory motifs (ITIMs) (1, 3). Mouse FCRL5 contains only five Ig-like domains in its ECD. It shares 49% aa sequence identity with human FCRL5 within common regions. Alternate splicing of human FCRL5 generates isoforms that consist of approximately the first one, six, or eight Ig-like domains (3, 4). FCRL5 expression is restricted to mature B lineage cells in lymphoid tissues and blood (3, 5‑7). Its ligation inhibits signaling through the B cell antigen receptor (8). Epstein-Barr virus transformation of B cells induces the up‑regulation of surface FCRL5 by a direct effect of its EBNA2 protein on FCRL5 gene transcription (9). The FCRL5 gene maps to the 1q21 chromosomal locus, a common site of rearrangements in B cell malignancies, and the FCRL5 protein is preferentially expressed in cell lines with 1q21 abnormalities (3). FCRL5 is up‑regulated on tumor cells in some types of B cell malignancies (6, 10‑12). In addition, soluble FCRL5 is elevated in the serum of many B cell leukemia patients (11, 13).

References
  1. Davis, R.S. (2007) Annu. Rev. Immunol. 25:525.
  2. Maltais, L.J. et al. (2006) Nat. Immunol. 7:431.
  3. Hatzivassiliou, G. et al. (2001) Immunity 14:277.
  4. SwissProt # Q96RD9.
  5. Miller, I. et al. (2002) Blood 99:2662.
  6. Polson, A.G. et al. (2006) Int. Immunol. 18:1363.
  7. Vidal-Laliena, M. et al. (2005) Cell. Immunol. 236:6.
  8. Haga, C.L. et al. (2007) Proc. Natl. Acad. Sci. 104:9770.
  9. Mohan, J. et al. (2006) Blood 107:4433.
  10. Ise, T. et al. (2005) Clin. Cancer Res. 11:87.
  11. Ise, T. et al. (2007) Leukemia 21:169.
  12. Kazemi, T. et al. (2009) Cancer Immunol. Immunother. 58:989.
  13. Ise, T. et al. (2006) Clin. Chem. Lab. Med. 44:594.
Long Name
Fc Receptor-like 5/3
Alternate Names
FCRL5/FCRL3

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human FCRL5/FCRL3 Antibody

There are currently no reviews for this product. Be the first to review Human FCRL5/FCRL3 Antibody and earn rewards!

Have you used Human FCRL5/FCRL3 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review