Human Fc gamma RIII (CD16) Biotinylated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: Fc gamma RIII (CD16)

CD16 (also known as FcgRIII) is a CD designation that, in human, encompasses two related, but distinct Ig superfamily genes; CD16a and CD16b. Mature human CD16a is a 45-75 kDa type I transmembrane glycoprotein that is 238 amino acids (aa) in length. It contains a 192 aa extracellular region that possesses two Ig-like domains plus glycosylation that is critical to its function, and ends with a short 25 aa cytoplasmic domain. On the cell surface, CD16a complexes with FceRI and CD3 zeta. CD16a shows a restricted expression pattern, being found on immature thymocytes, placental trophoblast cells, CD56dim and cytokine-activated CD56+ NK cells, slan (sulfated NAcLac)-expressing dendritic cells, and CX3CR1+ CD14+/- "patrolling" monocytes. Mature CD16b, by contrast, is slightly smaller that CD16a, but still shows the same 50-70 kSDa electrophoretic pattern in SDS-PAGE. It is a GPI-linked glycoprotein that is 184 aa in length. On the cell surface, CD16b is monomeric. The standard allele (= NA2) is encoded by the SwissProt Accession # used for this product (see above). The two variant alleles are NA1, which shows four aa substitutions, and SH, which contains only one aa substitution relative to the NA2 sequence. CD16b expression appears to be limited to neutrophils. CD16b is known to be solubilized, and the soluble form is believed to arise from the action of ADAM-17 rather than a phospholipase. Over aa 20-208, human CD16a and CD16b share 97% aa sequence identity. Over the same aa range, human CD16b and mouse CD16 share only 47% aa sequence identity.

Product Datasheets

Related Research Areas

• Common Macrophage Markers
• Dendritic Cell ITIM/ITAM Immunoreceptors
• Dendritic Cell Pathogen Recognition/Uptake
• Fc Receptors
• gamma delta T Cells
• Group 1 ILCs
• Inflammatory/Monocyte-derived Dendritic Cells
• Isolation, Culture, and Identification of Natural Killer Cells
• ITIM/ITAM Immunoreceptors and Related Molecules
• Leukocyte Receptors/Markers in Allergy and Asthma
• Lymphoid Lineage Markers
• Macrophage Activation Markers
• Microglia Activation
• Microglia Activation Markers
• Monocyte Markers
• Myeloid Lineage Markers
• Myeloid-derived Suppressor Cells (MDSC)
• Natural Killer T (NKT) Cells
• Neutrophils
• Uptake of Amyloid beta by Glia Cells