Human Fc gamma RIII (CD16) Alexa Fluor® 594-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2546T-100UG
R&D Systems Antibodies
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Human Fc gamma RIII (CD16) Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.
Source
Monoclonal Mouse IgG2a Clone # 245536
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Human whole blood granulocytes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Long Name
Fc gamma Receptor III
Entrez Gene IDs
14131 (Mouse); 102140945 (Cynomolgus Monkey)
Alternate Names
CD16; CD16A; Fc fragment of IgG receptor IIIa; Fc gamma RIII; FCG3; FCGR3; FcgRIII; FCR-10; FCRIII; FCRIIIA; IGFR3; IMD20

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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