Human Fas/TNFRSF6/CD95 Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Fas/TNFRSF6/CD95 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Fas/TNFRSF6/CD95 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF326) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Fas/TNFRSF6/CD95 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Fas/TNFRSF6/CD95
Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the new TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated
T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas. Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL‑mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.
- Nagata, S. and P. Golstein (1995) Science 267:1449.
- Nagata, S. (1997) Cell 88:355.
- Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
- Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
Product Datasheets
Citations for Human Fas/TNFRSF6/CD95 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 7
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Distinct immunocyte-promoting and adipocyte-generating stromal components coordinate adipose tissue immune and metabolic tenors
Authors: Raul German Spallanzani, David Zemmour, Tianli Xiao, Teshika Jayewickreme, Chaoran Li, Paul J. Bryce et al.
Science Immunology
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Endogenous IL-33 Is Highly Expressed in Mouse Epithelial Barrier Tissues, Lymphoid Organs, Brain, Embryos, and Inflamed Tissues: In Situ Analysis Using a Novel Il-33–LacZ Gene Trap Reporter Strain
Authors: Mélanie Pichery, Emilie Mirey, Pascale Mercier, Emma Lefrancais, Arnaud Dujardin, Nathalie Ortega et al.
The Journal of Immunology
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Characterizing the regulatory Fas (CD95) epitope critical for agonist antibody targeting and CAR-T bystander function in ovarian cancer
Authors: Mondal, T;Gaur, H;Wamba, BEN;Michalak, AG;Stout, C;Watson, MR;Aleixo, SL;Singh, A;Condello, S;Faller, R;Leiserowitz, GS;Bhatnagar, S;Tushir-Singh, J;
Cell death and differentiation
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Sex-specific adipose tissue imprinting of regulatory T cells
Authors: A Vasanthaku, D Chisanga, J Blume, R Gloury, K Britt, DC Henstridge, Y Zhan, SV Torres, S Liene, N Collins, E Cao, T Sidwell, C Li, RG Spallanzan, Y Liao, PA Beavis, T Gebhardt, N Trevaskis, SL Nutt, JD Zajac, RA Davey, MA Febbraio, D Mathis, W Shi, A Kallies
Nature, 2020-02-26;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
p53 enhances gefitinib-induced growth inhibition and apoptosis by regulation of Fas in non-small cell lung cancer.
Authors: Rho JK, Choi YJ, Ryoo BY, Na II, Yang SH, Kim CH, Lee JC
Cancer Res., 2007-02-01;67(3):1163-9.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
EphB4 provides survival advantage to squamous cell carcinoma of the head and neck.
Authors: Masood R, Kumar SR, Sinha UK, Crowe DL, Krasnoperov V, Reddy RK, Zozulya S, Singh J, Xia G, Broek D, Schonthal AH, Gill PS
Int. J. Cancer, 2006-09-15;119(6):1236-48.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas.
Authors: Legembre P, Moreau P, Daburon S, Moreau JF, Taupin JL
Cell Death Differ., 2002-03-01;9(3):329-39.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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A sandwich assay was developed using the polyclonal antibody AF326 as both capture and detection.
