Human Fas/TNFRSF6/CD95 Antibody

Catalog # Availability Size / Price Qty
MAB144
MAB144-SP
Product Details
Citations (2)
FAQs
Supplemental Products
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Human Fas/TNFRSF6/CD95 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Fas in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant human (rh) TNF RI, rhTNF RII, rmTNF RI, or rmTNF RII.
Source
Monoclonal Mouse IgG1 Clone # 50830
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fas
Gln26-Asn173
Accession # P25445
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Human Fas/TNFRSF6/CD95 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human Fas/TNFRSF6/CD95 Biotinylated Antibody (Catalog # BAF326)

Standard: Recombinant Human Fas/TNFRSF6/CD95 Fc Chimera Protein (Catalog # 326-FS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fas/TNFRSF6/CD95

Fas, also known as APO-1, CD95, and TNFRSF6, was originally identified as a cell-surface protein which binds to monoclonal antibodies that were cytolytic for various human cell lines. In the new TNF Receptor superfamily nomenclature, Fas is referred to as TNFRSF6. Human Fas cDNA encodes a 325 amino acid (aa) residue type 1 membrane protein that belongs to the TNF and NGF receptor family. Alternatively spliced cDNAs encoding multiple Fas isoforms, including a soluble form of Fas lacking the transmembrane domain, have also been identified. Fas is highly expressed in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes, and other tumor cells. Fas expression has also been detected in mouse thymus, liver, heart, lung, kidney and ovary. The ligand for Fas (FasL) has been identified and shown to be a member of the TNF family of type 2 membrane proteins. FasL is predominantly expressed by activated T‑lymphocytes, NK cells, and in tissues with immune-privileged sites. Soluble FasL can be produced by proteolysis of membrane-associated Fas.

Ligation of Fas by FasL or anti-Fas antibody has been shown to induce apoptotic cell death in Fas-bearing cells. Fas plays a role in the down-regulation of the immune reaction and has been shown to be a key mediator of activation-induced death of activated T‑lymphocytes. Fas-mediated cell death has also been shown to be important for the deletion of activated or autoreactive B‑lymphocytes. Besides the perforin/granzyme-based mechanism, the Fas system has been identified as the alternate pathway for CTL‑mediated cytotoxicity. FasL has also been shown to function in immunological privileged sites by killing infiltrating Fas-bearing lymphocytes and inflammatory cells.

References
  1. Nagata, S. and P. Golstein (1995) Science 267:1449.
  2. Nagata, S. (1997) Cell 88:355.
  3. Parijs, L. and A.K. Abbas (1996) Current Opinion in Immunol. 8:355.
  4. Green, D.R. and C.F. Ware (1997) Proc. Natl. Acad. Sci. USA 94:5986.
Long Name
Fibroblast-associated
Entrez Gene IDs
355 (Human); 14102 (Mouse); 246097 (Rat); 102140989 (Cynomolgus Monkey); 493881 (Feline)
Alternate Names
Apo-1 antigen; Apo-1; apoptosis antigen 1; Apoptosis-mediating surface antigen FAS; APT1; APT1FASTM; CD95 antigen; CD95; CD95ALPS1A; Fas (TNF receptor superfamily, member 6); Fas AMA; Fas antigen; Fas; FAS1; FASLG receptor; TNFRSF6; TNFRSF6member 6; tumor necrosis factor receptor superfamily member 6

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Citations for Human Fas/TNFRSF6/CD95 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulation of fibroblast Fas expression by soluble and mechanical pro-fibrotic stimuli
    Authors: AE Dodi, IO Ajayi, C Chang, M Beard, SL Ashley, SK Huang, VJ Thannickal, DJ Tschumperl, TH Sisson, JC Horowitz
    Respir. Res., 2018-05-10;19(1):91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  2. Regulation of the extrinsic apoptotic pathway by the extracellular matrix glycoprotein EMILIN2.
    Authors: Mongiat M, Ligresti G, Marastoni S, Lorenzon E, Doliana R, Colombatti A
    Mol. Cell. Biol., 2007-08-13;27(20):7176-87.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation

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