Human F-box protein 15/FBXO15 Antibody

Catalog #: AF6035 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6035
AF6035-SP

Detection of Human F-box protein 15/FBXO15 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human F-box protein 15/FBXO15 Antibody Summary

Species Reactivity

Human

Specificity

Detects human F‑box protein 15/FBXO15 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse F‑box protein 15/FBXO15 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human F‑box protein 15/FBXO15
aa 298-434
Accession # NP_689889

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human F-box protein 15/FBXO15 by Western Blot. Western blot shows lysates of human testis tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human F-box protein 15/FBXO15 Antibody Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6035) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for F-box protein 15/FBXO15 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: F-box protein 15/FBXO15

FBXO15 (F-box only protein 15; also FBX15) is a 50‑60 kDa member of the F-box family, O-subfamily of molecules that is most closely related to FBXO8 and O11. It is found in pluripotent stem cells and testis tissue, and apparently serves as a substrate recognition component of the SCF-type E3 ubiquitin ligase. Human FBXO15 is 434 amino acids (aa) in length. It contains one F-box (aa 1‑41) that likely serves as an interaction domain for SKP1. There are two potential isoforms, one that contains an alternative start site 76 aa upstream of the standard site, and a second that shows complex splicing that includes a 66 aa insertion after Asn35, a Ser substitution for aa 186‑256, and a 106 aa substitution for aa 304‑346. Over aa 298‑434, human FBXO15 shares 53% aa identity with mouse FBXO15.

Entrez Gene IDs

201456 (Human); 361354 (Rat)

Alternate Names

F-box only protein 15; Fbox protein 15; F-box protein 15; FBX15; FBX15MGC39671; FBXO15