Human Ephrin-A3 Antibody

Catalog #: MAB359 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB359
MAB359-SP

Detection of Human Ephrin‑A3 by Western Blot.

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Human Ephrin-A3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Ephrin-A3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human (rh) Ephrin-A5 is observed and no cross-reactivity with rhEphrin-A4, recombinant mouse (rm) Ephrin-A1, rmEphrin-B1, rhEphrin-B2, and rhEphrin-B3 is observed.

Source

Monoclonal Mouse IgG_2A Clone # 80814

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Ephrin-A3
Asn31-Ser209
Accession # AAA52368

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Ephrin‑A3 by Western Blot. Western blot shows lysates of IMR-32 human neuroblastoma cell line, A172 human glioblastoma cell line, U-118-MG human glioblastoma/astrocytoma cell line, and T47D human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Ephrin-A3 Monoclonal Antibody (Catalog # MAB359) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Ephrin-A3 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ephrin-A3

Ephrin-A3, also known as Ehk1-L, EFL-2, and LERK-3 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A3 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, EphA8, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A3 share 96% amino acid identity. Only membrane‑bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

Eph Nomenclature Committee [letter] (1997) Cell 90:403.
Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Entrez Gene IDs

1944 (Human)

Alternate Names

EFL2; EFL-2; EFNA3; Ehk1-L; EPH-related receptor tyrosine kinase ligand 3; EphrinA3; Ephrin-A3; EPLG3EHK1 ligand; LERK3; LERK3LERK-3; ligand of eph-related kinase 3