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Human EOMES Antibody

Catalog #: MAB6166
12 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6166
MAB6166-SP
Detection of Human EOMES by Western Blot.
3 Images
Product Details
Citations (12)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human EOMES Antibody Summary

Species Reactivity
Human
Specificity
Detects human EOMES in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Brachyury, rhEOMES (aa 1-115), recombinant mouse EOMES (aa 1-126), rhTBX2, 3, 5, 6, 18, or 20 is observed.
Source
Monoclonal Mouse IgG2B Clone # 644730
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human EOMES
Gly471-Pro686
Accession # O95936
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human EOMES antibody by Western Blot. View Larger

Detection of Human EOMES by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells untreated (-) or mesendoderm differentiated (+). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for EOMES at approximately 100 and 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EOMES antibody in Mesodermally Differentiated BG01V Human Stem Cells by Immunocytochemistry (ICC). View Larger

EOMES in Mesodermally Differentiated BG01V Human Stem Cells. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using Human EOMES Monoclonal Antibody (Catalog # MAB6166) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of EOMES antibody in Differentiated BG01VHuman Cells antibody by Flow Cytometry. View Larger

Detection of EOMES in Differentiated BG01VHuman Cells by Flow Cytometry. BG01V human embryonic stem cells differentiated to mesendoderm were stained with Mouse Anti-Human EOMES Monoclonal Antibody (Catalog # MAB6166, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allo-phycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EOMES

EOMES (Eomesodermin; Eo from Greek meaning “dawn”/early in mesoderm; also TBR2) is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. 

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

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Citations for Human EOMES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. STRADA‐mutant human cortical organoids model megalencephaly and exhibit delayed neuronal differentiation
    Authors: Louis T. Dang, Shivanshi Vaid, Grace Lin, Preethi Swaminathan, Jordan Safran, Anna Loughman et al.
    Developmental Neurobiology
  2. Development and Characterization of Human Cerebral Organoids: An Optimized Protocol
    Authors: Abraam M. Yakoub, Mark Sadek
    Cell Transplantation
  3. Human 3D cellular model of hypoxic brain injury of prematurity
    Authors: Wada H, Ikoma K, Oka Y et al.
    Nat. Med.
  4. Tumor suppressor SMARCB1 suppresses super-enhancers to govern hESC lineage determination
    Authors: Lee F Langer, James M Ward, Trevor K Archer
    eLife
  5. Endogenous FGFs drive ERK-dependent cell fate patterning in 2D human gastruloids
    Authors: Jo, K;Liu, ZY;Patel, G;Yu, Z;Yao, L;Teague, S;Johnson, C;Spence, J;Heemskerk, I;
    bioRxiv : the preprint server for biology
    Species: Human
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  6. A simple method to improve the quality and yield of human pluripotent stem cell-derived cerebral organoids
    Authors: MS Choe, SJ Kim, ST Oh, CM Bae, WY Choi, KM Baek, JS Kim, MY Lee
    Heliyon, 2021-06-19;7(6):e07350.
    Species: Human
    Sample Types: Organoid
    Applications: ICC
  7. Primate cell fusion disentangles gene regulatory divergence in neurodevelopment
    Authors: RM Agoglia, D Sun, F Birey, SJ Yoon, Y Miura, K Sabatini, SP Pa?ca, HB Fraser
    Nature, 2021-03-17;0(0):.
    Species: Human, Primate
    Sample Types: Organoid
    Applications: ICC
  8. Endoderm and Hepatic Progenitor Cells Engraft in the Quiescent Liver Concurrent with Intrinsically Activated Epithelial-to-Mesenchymal Transition
    Authors: WS Fagg, N Liu, I Patrikeev, OA Saldarriag, M Motamedi, VL Popov, HL Stevenson, JH Fair
    Cell Transplantation, 2021-01-01;30(0):9636897219937.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Reliability of human cortical organoid generation
    Authors: SJ Yoon, LS Elahi, AM Pa?ca, RM Marton, A Gordon, O Revah, Y Miura, EM Walczak, GM Holdgate, HC Fan, JR Huguenard, DH Geschwind, SP Pa?ca
    Nat. Methods, 2018-12-20;16(1):75-78.
    Species: Human
    Sample Types: Organoids
    Applications: IHC
  10. WNT signaling memory is required for ACTIVIN to function as a morphogen in human gastruloids
    Authors: A Yoney, F Etoc, A Ruzo, T Carroll, JJ Metzger, I Martyn, S Li, C Kirst, ED Siggia, AH Brivanlou
    Elife, 2018-10-12;7(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks.
    Authors: Latos P, Sienerth A, Murray A, Senner C, Muto M, Ikawa M, Oxley D, Burge S, Cox B, Hemberger M
    Genes Dev, 2015-11-19;29(23):2435-48.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Myeloid antigen-presenting cell niches sustain antitumor T cells and license PD-1 blockade via CD28 costimulation
    Authors: Jaikumar Duraiswamy, Riccardo Turrini, Aspram Minasyan, David Barras, Isaac Crespo, Alizée J. Grimm et al.
    Cancer Cell

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Reviews for Human EOMES Antibody

Average Rating: 4.5 (Based on 2 Reviews)

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Human EOMES Antibody
By Anonymous on 09/04/2021
Application: WB Sample Tested: Embryonic stem cell derived mesoderm Species: Human
Western Blot Human EOMES Antibody MAB6166
Human EOMES Antibody
By Anonymous on 06/22/2016
Application: WB Sample Tested: Embryonic stem cell derived mesoderm Species: Human
Western Blot Human EOMES Antibody MAB6166